Serum-Free Medium Powder for MDCK, VERO and BHK-21

CCM-SF-0042 is a serum-free medium dry powder in the serum-free media portfolio, supplied for preparation of 50 L of finished culture medium. The source formulation is intended for use with 2% fetal bovine serum added by the user, so procurement teams should classify it as a serum-free base powder used in a reduced-serum process rather than as a fully serum-absent final culture system. This distinction is important for bill-of-material control, incoming QC, and media-preparation SOPs.

The powder is specified as animal-origin-free before user supplementation and includes 10 μg/L EGF, 584.6 mg/L L-glutamine, and sodium bicarbonate. Target finished-medium specifications are pH 7.0-7.6 and osmolality 280-350 mOsmol/kg. Endotoxin is specified at ≤3 EU/mL. The culture environment is 37 °C with 5-10% CO2, supporting common adherent and suspension-adapted development work across virology research, cell banking studies, upstream screening, and scale-up evaluation. For controlled sourcing, teams may submit forecast quantities through the request quote page.

• Culture and expansion of MDCK, VERO, Marc 145, ST, PK-15, MDBK, HEp2, and BHK-21 cell lines for research and bioprocess development programs.
• Serum-reduced media optimization where a dry powder base is prepared and supplemented with 2% fetal bovine serum according to a validated internal protocol.
• Upstream process characterization requiring defined targets for pH, osmolality, endotoxin, and controlled storage life.
• Seed-train, shake flask, roller bottle, multilayer vessel, and small bioreactor studies where consistent medium preparation and lot traceability are required.
• Procurement consolidation for organizations standardizing powder media formats to reduce shipping volume, improve storage efficiency, and support multi-site preparation.

Store unopened CCM-SF-0042 powder at 2-8 °C protected from light. The stated shelf life is 24 months when stored under recommended conditions and handled within approved quality procedures. Shipments are arranged on blue ice to maintain cold-chain integrity during transit. During preparation, use qualified water, controlled mixing, and validated aseptic handling practices. Add fetal bovine serum at 2% only where it is required by the site protocol, and confirm final pH and osmolality before release to cell culture. Because the medium contains L-glutamine and sodium bicarbonate, avoid unnecessary warming, repeated container opening, and extended exposure to ambient light or air during preparation.

CellCultureMedia supplies CCM-SF-0042 under ISO-aligned supplier controls with procurement documentation suitable for R&D, process development, and non-commercial bioprocess workflows. Lot documentation and Certificate of Analysis are available on request, including applicable release data such as pH, osmolality, endotoxin, appearance, and lot identification. Sterility-related handling should be governed by the buyer’s validated preparation and filtration procedure unless otherwise stated in lot-specific documentation. For supplier qualification, quality questionnaires, or documentation review, visit quality documentation. Free worldwide shipping is available, and bulk quotations can include multi-unit pricing, scheduled releases, and shipment coordination.