CHO Serum-Free Medium Powder, 50 L

CCM-SF-0004 is a chemically defined, serum-free medium powder configured for CHO cell culture processes that require animal component-free input materials. It is supplied in a 50 L manufacturing pack for users who need consistent lot-to-lot handling, defined supplementation control, and simplified inbound logistics. The powder format supports centralized preparation under the buyer’s qualified water, filtration, mixing, and release procedures. It is suitable for teams comparing serum-free media options within the serum-free media category.

The specification profile includes animal origin-free classification, powder form, D-glucose included, and no L-glutamine or HT supplement supplied in the base formulation. The medium is designed for incubation at 37℃ with 5–10% CO2, depending on the cell line and process set point. Lot specifications include endotoxin at ≤3 EU/mL, osmolality of 260–300 mOsmol/kg, and pH range of 3.4–4.0 as specified for the supplied product. Because the additive data indicate no phenol red, buyers requiring a color-indicator configuration should confirm the final release configuration at quote stage.

• Serum-free suspension culture of CHO K1, CHO-S, CHO-GS, and CHO DG44 cells in development and scale-up workflows.
• Animal component-free upstream process development where controlled raw-material origin and defined supplementation are required.
• Screening and adaptation studies for recombinant protein expression, vaccine process research, and biopharmaceutical manufacturing development.
• Media preparation programs where L-glutamine is added separately to control timing, concentration, and degradation profile.
• Comparability work between shake flask, spinner, benchtop bioreactor, and larger stirred-tank formats, subject to site validation.
• Procurement standardization for laboratories that require a 50 L powder format rather than multiple smaller liquid media units.

Store unopened material at 2–8℃ and protect from light. The product ships on blue ice to support temperature-controlled receipt, and free worldwide shipping is available where import and routing conditions allow. After receipt, transfer promptly to qualified cold storage and keep the container sealed until use. Reconstitute only under validated site procedures using qualified water and mixing equipment, and add L-glutamine or HT supplement separately if required by the user’s CHO process. As with all powder media, avoid moisture ingress, repeated temperature excursions, and uncontrolled exposure during weighing or transfer.

CellCultureMedia supplies CCM-SF-0004 for research, cell culture, and bioprocessing use under ISO-aligned quality and supplier management practices. Lot-specific documentation can be requested, including Certificate of Analysis details for endotoxin, osmolality, pH, formulation identity, and other release attributes available for the lot. Procurement teams may review documentation expectations through our quality information page before ordering. For budgetary planning, the 50 L pack is listed at $15,080.56; volume pricing, delivery routing, and document requirements can be coordinated through request a quote.