Serum-Free CHO Medium, ADCF, Phenol Red

CCM-SF-0002 is a ready-to-use liquid CHO medium designed for research and bioprocess development environments that require defined, serum-free, animal-origin-free culture conditions. The formulation contains phenol red for visual pH indication and D-glucose as a principal carbohydrate source. It does not contain L-glutamine or HT supplement, giving process engineers flexibility to add fresh glutamine, stable glutamine alternatives, hypoxanthine-thymidine, or other supplements at defined concentrations when required by the cell line or selection strategy.

The medium is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 workflows, including platform screening, inoculum expansion, clone evaluation, and upstream process optimization. Typical culture conditions are 37 °C with 5–10% CO2, subject to site-specific vessel geometry, gas transfer, feed strategy, and cell-line adaptation status. Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterile-filtered liquid presentation. The 500 mL bottle format is suitable for development laboratories that need lot-controlled material without committing immediately to large-volume procurement. For larger requirements, buyers can submit planned volumes through request a quote for contract pricing and supply planning.

• CHO platform development: supports work with CHO K1, CHO-S, CHO-GS, and CHO DG44 cells where serum-free, animal-derived component-free conditions are required for consistency and simplified downstream impurity profiles.
• Clone and media screening: suitable for comparing growth, viability, metabolite behavior, and productivity across clones or adapted sublines, particularly where the absence of L-glutamine and HT supplement is used to standardize controlled-addition experiments.
• Seed train and inoculum studies: can be used in shake flasks, spinner systems, and small-scale bioreactors when the process is qualified for the medium and the recommended CO2 and temperature range is maintained.
• Biopharmaceutical process research: applicable to upstream development for recombinant protein and antibody expression workflows, including studies where defined medium composition, low endotoxin specification, and sterile-filtered supply are procurement requirements.
• Scale-up evaluation: the osmolality range of 270–340 mOsmol/kg and pH range of 7.0–7.4 provide defined baseline parameters for evaluating feed timing, pH control, lactate behavior, and cell-specific productivity under controlled bench-scale conditions.

Store CCM-SF-0002 at 2–8 °C protected from light. Do not freeze, because freezing can affect liquid medium performance and may cause precipitation or container stress. The product ships on blue ice to help maintain refrigerated transit conditions, and receiving teams should transfer bottles to qualified cold storage promptly after delivery. Before use, inspect the bottle for leakage, turbidity, color shift outside the expected phenol red range, or visible particulates. Warm only the required volume to the working temperature and minimize repeated warming cycles. Aseptic handling is required after opening, even though the product is supplied sterile-filtered. Because L-glutamine and HT supplement are not included, any required additions should be made using qualified sterile components and recorded in the batch or experiment documentation. Shelf life is controlled by the lot-specific label and supporting documentation rather than a generic open-ended period.

CellCultureMedia supplies CCM-SF-0002 for B2B research, process development, and bioprocessing use with specification-oriented documentation to support procurement review. Manufacturing and release controls are managed under ISO-aligned quality practices, with attention to sterile filtration, traceable lot release, and defined physicochemical specifications. A Certificate of Analysis is available on request and typically includes lot identity, pH, osmolality, endotoxin, sterility status, and other release attributes relevant to the product. Additional quality or regulatory support questions can be routed through our quality documentation channel before purchase order placement. The listed 500 mL price is USD 319.44, and free worldwide shipping is available for standard orders, subject to destination import requirements and cold-chain feasibility.