Serum-Free CHO Medium Set

CCM-SF-0001 is a serum-free CHO medium set supplied in liquid form for animal origin-free mammalian cell culture. It is intended for organizations developing, qualifying, or scaling CHO-based expression systems where defined culture inputs, lot traceability, and consistent procurement specifications are important. The formulation is sterile-filtered and suitable for use under standard CHO incubation conditions of 37 °C with 5-10% CO2.

This product is positioned within the serum-free media portfolio for procurement teams that need a ready-to-use medium set rather than a dry powder format or a serum-containing system. It supports CHO K1, CHO-S, CHO-GS, and CHO DG44 workflows, including clone evaluation, medium screening, seed train development, and protein expression process studies. The animal origin-free classification helps reduce variability associated with undefined animal-derived components and supports more controlled raw-material qualification programs.

• CHO cell culture development: designed for serum-free growth and maintenance studies using CHO K1, CHO-S, CHO-GS, and CHO DG44 cells in controlled incubator environments.
• Upstream process development: appropriate for shake flask, small-bioreactor, and scale-down model work where process engineers need liquid media with defined handling and storage parameters.
• Expression workflow evaluation: suitable for biopharmaceutical and vaccine-related research programs that use CHO host systems for recombinant protein or particle production research.
• Serum-reduction and serum-free conversion: can be evaluated as part of adaptation studies for laboratories transitioning CHO cultures away from serum-containing conditions.
• Procurement standardization: useful for teams consolidating serum-free media purchasing under documented specifications, single-SKU quotation, and repeatable ordering through request-for-quote workflows.

Store CCM-SF-0001 at 2-8 °C and protect the container from unnecessary warming during receipt, staging, and routine use. The product ships on blue ice to maintain refrigerated transit conditions and has a stated shelf life of 12 months when stored as recommended and kept unopened. Use aseptic technique when connecting, pouring, or aliquoting the liquid medium, and avoid repeated temperature cycling after opening. Before use, equilibrate only the required volume to the intended culture temperature, inspect for container integrity and visible particulates, and follow the facility’s approved cell-culture handling procedure. Do not freeze unless a validated site-specific procedure supports that practice.

CellCultureMedia supplies CCM-SF-0001 for research and bioprocessing customers that require specification-driven ordering and dependable documentation. Manufacturing and release controls are managed in an ISO-aligned quality framework, with sterile filtration as the stated sterility approach. A lot-specific Certificate of Analysis is available on request, and additional documentation can be coordinated through the quality documentation process. Where released for the lot, documentation may include identity, sterility, appearance, and other physicochemical test data relevant to medium qualification. Free worldwide shipping is available for eligible orders, and procurement teams may request commercial quotes for repeat supply, standing orders, or multi-site purchasing programs.