Serum-Free Medium for 293 Suspension Cells, 500 mL

CCM-SF-0061 is a sterile-filtered, serum-free liquid medium for 293 suspension cells used in recombinant protein expression, viral vector process development, transfection optimization, and upstream bioprocess screening. It is supplied ready for use and does not require serum supplementation. The medium includes phenol red for visual pH monitoring, D-glucose as a carbon source, and 4 mM L-glutamine to support nitrogen metabolism in actively growing 293 suspension cultures.

The product is classified as animal-origin and should be evaluated against each site’s raw material policy before use in regulated manufacturing pipelines. Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin ≤3 EU/mL, and sterility by filtration. For buyers comparing serum-free options, this item belongs in the serum-free media category and is configured for standard humidified incubator conditions at 37 °C with 5–10% CO2.

• Suspension culture of HEK293-derived cell lines, including 293F-type systems and comparable high-density 293 workflows.
• Serum-free adaptation studies where process teams need to reduce lot-to-lot variability associated with serum-containing formulations.
• Transient transfection development for plasmid-based expression studies, with medium compatibility to be confirmed using the site’s selected transfection reagent and feed plan.
• Upstream process screening for vaccine and biopharmaceutical research programs, including shake flask, spinner, and small bioreactor formats.
• Routine R&D culture maintenance when phenol red indication, defined L-glutamine content, and documented pH and osmolality ranges are required for batch records.

Store CCM-SF-0061 at 2–8 °C protected from light. Do not freeze unless your internal qualification protocol has confirmed acceptable performance after freeze-thaw exposure. The stated shelf life is 12 months when stored under the recommended conditions in the original, unopened container. The product ships on blue ice to maintain refrigerated transit conditions. Before use, bring the bottle to the required working temperature using controlled warming, mix gently, and apply aseptic technique when opening, sampling, or transferring to culture vessels.

CellCultureMedia supplies CCM-SF-0061 for research, development, and bioprocessing use with ISO-aligned quality practices, sterile filtration, and lot-level specification control. A Certificate of Analysis is available on request and can support incoming QC review for pH, osmolality, endotoxin, sterility, shelf life, and lot identification. Additional quality documentation requests can be submitted through our quality page. For multi-bottle purchases, standing supply, or site qualification quantities, contact procurement through request quote; eligible orders include free worldwide shipping.