Serum-Free CHO Powder Medium for CHO Cell Culture

This serum-free CHO powder medium is designed for animal origin-free culture workflows where defined composition, scalable preparation, and lot documentation are required. It is supplied as a dry powder for preparation of 50 L of finished medium and is suitable for organizations standardizing CHO process development, recombinant protein expression studies, and upstream bioprocess screening. The formulation is phenol red-free to support optical readouts and assay designs where background color interference should be minimized. It is also supplied without L-glutamine, sodium bicarbonate, and HT supplement so process teams can select validated concentrations and addition timing for their own platform.

Key release specifications include serum-free classification, animal origin-free classification, osmolality of 300–340 mOsmol/kg, pH level of 3.3–3.9, and endotoxin not more than 3 EU/mL. Recommended culture conditions are 37°C with 5–10% CO2, depending on vessel format, bicarbonate adjustment, and local process controls. The product is categorized under serum-free media and is aligned with CHO K1, CHO-S, CHO-GS, and CHO DG44 culture requirements. The listed pack size is 50 L, with a published price of USD 15,080.56 for procurement reference.

• Serum-free expansion and maintenance of CHO K1, CHO-S, CHO-GS, and CHO DG44 cell lines in suspension culture workflows.
• Research and process development for recombinant protein and biopharmaceutical production platforms requiring animal origin-free media inputs.
• Screening studies where users need independent control of L-glutamine, sodium bicarbonate, and HT supplementation.
• Phenol red-free culture conditions for analytical workflows where colorimetric interference or absorbance background must be reduced.
• Scale-up planning from shake flask and bench-scale bioreactor studies to larger controlled upstream runs, subject to site-specific qualification.
• Procurement programs that require defined shelf life, endotoxin limits, osmolality range, and documentation traceability before purchase approval.

Store the unopened powder at 2–8°C protected from light. The stated shelf life is 24 months when maintained under recommended storage conditions and original packaging integrity is preserved. Shipment is made on blue ice to support temperature-controlled transit. After receipt, inspect packaging, record lot details, and return material to refrigerated storage promptly. Prepare medium using validated site procedures, suitable water quality, and sterile filtration or aseptic processing steps as required by the end user. Because sodium bicarbonate, L-glutamine, and HT supplement are not included, add only qualified supplements according to the process-specific formulation and confirm pH, osmolality, and appearance before use.

CellCultureMedia supplies CCM-SF-0013 for research and bioprocessing organizations that require consistent sourcing, clear specifications, and responsive commercial support. Manufacturing and release controls are managed under ISO-aligned quality practices, with lot-specific documentation available to support internal review. A Certificate of Analysis can be requested through Quality and Documentation, including release attributes such as endotoxin, osmolality, pH, classification, and shelf life where applicable. For formal pricing, standing orders, forecasted demand, or multi-site supply planning, procurement teams may use the request quote channel. Free worldwide shipping is available, with blue-ice logistics used for this refrigerated powder format.