Serum-Free CHO Medium with Phenol Red and HT

CCM-SF-0005 is designed for CHO K1, CHO-S, CHO-GS, and CHO DG44 workflows where serum-free, animal-derived component-free media are required for consistent cell culture operations. The formulation is supplied as a ready-to-use liquid and includes phenol red for visual pH monitoring, D-glucose as the principal carbohydrate source, and HT supplement to support hypoxanthine-thymidine dependent culture strategies. L-glutamine is intentionally omitted, allowing process engineers to control the timing, concentration, and chemistry of glutamine supplementation according to the cell line and feeding plan.

This medium is suitable for procurement programs that standardize raw materials for R&D, process development, and scale-up studies. The listed release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, sterile filtration, and endotoxin not more than 3 EU/mL. Each 500 mL unit is priced at $319.44. Related media options can be reviewed in our serum-free media category, or teams can submit a volume forecast through request quote support for multi-unit purchasing.

• Serum-free culture of CHO K1, CHO-S, CHO-GS, and CHO DG44 cell lines in incubators maintained at 37°C with 5-10% CO2.
• Process development studies requiring animal-derived component-free media to reduce lot-to-lot variability associated with serum-containing systems.
• Suspension CHO workflows for recombinant protein expression, cell line evaluation, media screening, feed strategy development, and upstream bioprocess optimization.
• Laboratory and pilot-scale biopharmaceutical research programs where defined media composition, documented quality attributes, and reproducible handling requirements are important for procurement approval.
• Culture systems where users prefer to add L-glutamine, glutamine dipeptide, or other supplements separately based on internal protocols, stability requirements, or feeding schedules.

Store CCM-SF-0005 at 2-8°C protected from light. Do not freeze unless the material has been evaluated under an internal stability protocol, because freeze-thaw exposure may affect solubility, appearance, or performance. Handle using aseptic technique, and minimize bottle-open time to preserve sterility after first use. The medium is supplied sterile-filtered and should be equilibrated to the intended culture temperature only for the working volume needed. Because L-glutamine is not included, any glutamine or dipeptide supplement should be added using sterile components and documented concentration targets. Shipments are prepared on blue ice to maintain refrigerated conditions in transit.

CellCultureMedia supplies CCM-SF-0005 for research and bioprocessing use under ISO-aligned supplier controls, incoming material review, and batch release documentation. A Certificate of Analysis can be requested to confirm lot-specific attributes such as appearance, pH, osmolality, sterility status, and endotoxin specification. Procurement teams can review additional supplier and documentation expectations through our quality information page. Standard orders include free worldwide shipping where available, with blue-ice packaging selected for temperature-sensitive delivery. For recurring use, bulk orders, or standing delivery schedules, include the CCM-SF-0005 SKU, required quantity, destination, and requested delivery window when contacting the commercial team.