Serum-Free Medium with Phenol Red and L-Glutamine

CCM-SF-0039 is supplied as a ready-to-use liquid medium for serum-free media workflows in research, process development, and biopharmaceutical cell culture. The formulation includes phenol red for visual pH indication, D-glucose as a carbohydrate source, and 4 mM L-glutamine for nitrogen and energy metabolism. It is manufactured as an animal-origin-free medium prior to customer-added supplements; because the specified protocol requires 2% fetal bovine serum, the final prepared culture system should be considered low-serum rather than animal-origin-free.

Key release specifications include pH 7.0–7.4, osmolality 280–320 mOsmol/kg, endotoxin ≤3 EU/mL, and filter-sterilized status. These parameters support procurement comparison, incoming quality review, and scale-up planning for laboratories standardizing across serum-free media and reduced-serum culture conditions. The 500 mL pack size is suitable for cell line screening, seed train development, and pilot-scale evaluations where consistent formulation and documented lot traceability are required.

• Culture of MDCK, VERO, Marc 145, ST, PK-15, MDBK, HEp2, and BHK-21 cell lines under 37 °C incubation with 5–10% CO2.
• Research and process-development workflows requiring a liquid medium with phenol red, D-glucose, and 4 mM L-glutamine already included.
• Viral production, antigen expression, and biopharmaceutical upstream development where the process calls for a defined medium base plus 2% fetal bovine serum.
• Media qualification studies comparing pH, osmolality, endotoxin, and cell growth performance across lots or across multiple adherent cell platforms.
• Procurement programs that need a documented 500 mL product format, clear storage requirements, and quotation support through the request quote workflow.

Store CCM-SF-0039 at 2–8 °C protected from light. Do not freeze unless a validated internal procedure has been established, as freezing can affect component solubility and appearance. Bring the bottle toward working temperature using controlled laboratory practice, mix gently before use, and handle aseptically after opening. The product is shipped on blue ice and has a stated shelf life of 12 months when stored unopened under the recommended conditions. Because L-glutamine is included, minimize prolonged warming and repeated temperature cycling during routine use.

CellCultureMedia supplies CCM-SF-0039 for research and bioprocessing use with specification-driven documentation for purchasing, receiving inspection, and technical review. Manufacturing and release controls are ISO-aligned, and lot documentation can include Certificate of Analysis information for pH, osmolality, endotoxin, and sterility testing where available; documentation requests can be routed through quality support. The listed price is $319.44 per 500 mL bottle, with free worldwide shipping offered where route, temperature-control, and customs requirements allow. For recurring demand, multi-bottle ordering, or program supply planning, CellCultureMedia can quote volume pricing and expected dispatch timing before purchase order placement.