Serum-Free AOF CHO Powder Medium

This powdered CHO medium is designed for serum-free suspension culture where procurement teams need animal-origin-free classification, lot documentation, and controlled reconstitution at site scale. It is part of the CellCultureMedia serum-free media portfolio and is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 cell culture platforms. The powder format reduces shipping volume and supports centralized media preparation for R&D, process development, and pilot-scale workflows.

The formulation is phenol red-free, which is useful when colorimetric interference must be minimized or when pH monitoring is managed through validated probes, analyzers, or offline sampling. L-glutamine and HT supplement are not included, allowing users to add these components separately according to process-specific stability requirements. D-glucose is included in the formulation. When prepared according to the applicable instructions, the specified pH range is 5.6 to 6.2, osmolality is 260 to 300 mOsmol/kg, and endotoxin is not more than 3 EU/mL. Recommended culture conditions are 37 °C with 5–10% CO2, subject to local cell-line adaptation and process controls.

• Serum-free expansion and maintenance of compatible CHO host-cell systems, including CHO K1, CHO-S, CHO-GS, and CHO DG44.
• Animal-origin-free media preparation for recombinant protein expression, clone screening, fed-batch development, and upstream process optimization.
• Phenol red-free culture conditions for workflows requiring reduced optical background or independent pH measurement.
• Media-preparation programs where L-glutamine and HT supplement are added separately to match internal formulation controls.
• Biopharmaceutical process development, academic research, and contract laboratory supply planning. For volume requirements, use the request quote page to confirm availability, documentation, and account pricing.

Store the unopened powder at 2–8 °C and protect it from light. The stated shelf life is 24 months when the product is stored under the recommended conditions and the original packaging remains intact. Shipments are handled on blue ice where required to support temperature-controlled transit, and free worldwide shipping is available for eligible orders. Reconstitute using qualified water and validated mixing, filtration, and addition procedures established by your facility. Because this medium is supplied without L-glutamine and HT supplement, add those components only if required by the selected CHO process, and document final concentrations in the batch record.

CellCultureMedia supplies CCM-SF-0019 for professional cell culture procurement with ISO-aligned supplier controls and lot-level release documentation. Key release specifications include powder form, serum-free composition, animal-origin-free classification, pH 5.6–6.2, osmolality 260–300 mOsmol/kg, endotoxin ≤3 EU/mL, and a 24-month shelf life under recommended storage. A Certificate of Analysis can be requested for the relevant lot, and sterility-related documentation can be reviewed where included in the release package. Visit quality documentation for supplier quality expectations or contact CellCultureMedia before purchase if your team needs additional compliance files, shipping notes, or procurement support.