Serum-Free AOF CHO Medium with Phenol Red

CCM-SF-0010 is a sterile-filtered, liquid serum-free CHO medium designed for animal-origin-free culture systems where defined composition, lot traceability, and reduced serum variability are procurement requirements. The formulation includes phenol red for visual pH indication and D-glucose as a primary carbon source, while excluding L-glutamine to give users control over glutamine supplementation strategy, timing, and concentration. It is suitable for laboratories and process groups standardizing CHO K1, CHO-S, CHO-GS, or CHO DG44 workflows in shake flasks, spinner vessels, and compatible bioreactor development platforms.

The medium is specified at pH 7.0-7.4 and osmolality 270-340 mOsmol/kg, supporting controlled development work at 37℃ in 5-10% CO₂ culture conditions. Endotoxin is specified at ≤3 EU/mL, and the product is supplied sterile-filtered for direct use under aseptic technique. As part of the CellCultureMedia serum-free media portfolio, this item is positioned for buyers who need consistent 500 mL liquid supply, transparent release specifications, and documentation suitable for internal raw-material qualification.

• Serum-free expansion and maintenance of CHO K1, CHO-S, CHO-GS, and CHO DG44 cell cultures used in recombinant protein expression and biologics process development.
• Animal-origin-free culture model setup where teams want to reduce undefined animal-derived inputs while retaining a liquid, ready-to-use base medium with phenol red monitoring.
• Media screening studies comparing feed timing, glutamine supplementation, glucose management, and osmolality response across suspension CHO platforms.
• Small-scale to pilot-scale upstream development in shake flasks, tube cultures, spinner systems, and controlled bioreactor runs operated at 37℃ and 5-10% CO₂.
• Procurement standardization for academic, biotech, and biopharma groups that require consistent specifications across repeat purchases, including pH, osmolality, sterility, endotoxin, storage, and shelf-life criteria.
• Workflows where L-glutamine is intentionally added separately, replaced with a stabilized glutamine source, or omitted during defined experimental comparisons. Users should validate supplementation according to their cell line, inoculation density, target passage range, and productivity objectives.

For multi-bottle ordering, standing demand, or site-level stocking, buyers can use the request a quote workflow to confirm volume pricing, packaging preferences, and destination-specific shipping details.

Store CCM-SF-0010 at 2-8℃ protected from light. Do not freeze, and minimize repeated warming cycles to help maintain formulation consistency throughout the labeled 12-month shelf life. The product ships on Blue Ice to support refrigerated transit conditions; inspect the container on receipt and place it at 2-8℃ promptly. Before use, mix gently by inversion, avoid foaming, and warm only the volume required for the procedure. Because the formulation is supplied without L-glutamine, add any required glutamine or alternative supplement aseptically immediately before use, following internally qualified concentrations. Use sterile technique with biosafety cabinet handling, sterile transfer devices, and appropriate in-process controls for pH, cell density, viability, and osmolality.

CellCultureMedia supplies this serum-free CHO medium under ISO-aligned manufacturing and quality management practices, with release testing focused on identity, appearance, pH, osmolality, sterility, endotoxin, and shelf-life assignment. A lot-specific Certificate of Analysis is available on request for quality review, supplier qualification, and incoming material records. Additional documentation requests can be routed through the CellCultureMedia quality team. Free worldwide shipping is available for eligible orders, and packaging is selected to maintain cold-chain integrity during transit. For regulated internal workflows, customers should qualify each lot in their own cell culture system before routine production use.