Reduced-Serum High-Glucose DMEM with NEAA and Pyruvate

CCM-SF-0063 is an advanced reduced-serum DMEM designed for research and process-development groups that need a defined base for lowering serum input while maintaining familiar high-glucose DMEM chemistry. The formulation contains phenol red for visual pH monitoring, 4.5 g/L D-glucose for energy-intensive cultures, 0.11 g/L sodium pyruvate as an additional carbon source, and MEM non-essential amino acids to reduce metabolic burden during expansion. It is manufactured as a liquid, animal-origin classified medium for scientific and basic research use.

The medium is specified for culture at 37 °C in 5–10% CO2 and is intended for systems supplemented with 1–5% FBS. Key release specifications include pH 7.0–7.4, osmolality 320–350 mOsmol/kg, endotoxin ≤1 EU/mL, and sterile-filtered status. L-glutamine and HEPES are intentionally excluded, allowing laboratories to add fresh glutamine or alternative buffering components according to internal protocols. For adjacent reduced-serum and chemically controlled options, review the serum-free and reduced-serum media category.

• Reduced-serum maintenance and expansion of fibroblasts, smooth muscle cells, and comparable adherent cell types in research environments.
• Protocol development where teams are transitioning from conventional serum-containing DMEM toward lower-serum culture conditions using 1–5% FBS.
• Comparability studies requiring high-glucose DMEM with phenol red, sodium pyruvate, and MEM non-essential amino acids, but without pre-added L-glutamine.
• Process engineering evaluations where osmolality, pH range, endotoxin limit, and sterile-filtered liquid format must be documented for batch records.
• Academic, biotech, and biopharma R&D workflows that require consistent 500 mL packaging, blue-ice logistics, and procurement-ready specifications.

Store CCM-SF-0063 at 2–8 °C protected from light. Do not freeze unless a validated internal procedure has been established, as freezing may affect solubility or container integrity. Warm only the required volume to room temperature or the validated process temperature before use, and handle aseptically in a qualified biosafety cabinet. Because L-glutamine is not included, add glutamine or a stable substitute only when required by the cell line and experimental design. The stated shelf life is 12 months when unopened and stored under the recommended conditions.

CellCultureMedia supplies CCM-SF-0063 for research and bioprocessing procurement teams that require lot-level traceability and clear release criteria. Manufacturing and quality controls are ISO-aligned, and a Certificate of Analysis can be requested through quality documentation support. Typical documentation may confirm appearance, pH, osmolality, sterility status, endotoxin limit, shelf life, storage range, and shipping condition. For volume requirements, standing supply, or multi-site purchasing, submit a request through request a quote. Free worldwide shipping is available where applicable, with temperature-controlled dispatch on blue ice.