Reduced Serum DMEM/F-12 Medium, 500 mL

CCM-SF-0064 is a reduced-serum DMEM/F-12 (1:1) formulation for laboratories that require a controlled nutrient base while limiting serum input to 1–5% FBS. The medium is supplied as a liquid, sterile-filtered product in a 500 mL format for research and bioprocess development use. It contains phenol red for visual pH indication, 3.15 g/L D-glucose as the carbohydrate source, sodium pyruvate for additional metabolic flexibility, and MEM non-essential amino acids to reduce cellular biosynthetic burden during routine culture.

The formulation is supplied without L-glutamine and without HEPES, allowing process teams to add these components separately when required by a cell line, feeding plan, or stability strategy. Recommended culture conditions are 37°C with 5–10% CO2, with a release pH range of 7.0–7.4. The product is classified as animal origin because it is designed for use with low FBS supplementation. Endotoxin is specified at ≤1 EU/mL, sterility is controlled by filtration, and osmolality is managed by lot within the finished medium release program.

• Low-serum expansion and maintenance of neuronal, glial, endothelial, and other mammalian cell cultures where 1–5% FBS is appropriate.
• Comparative studies evaluating reduced serum input against conventional DMEM/F-12 systems while retaining phenol red, sodium pyruvate, and non-essential amino acid supplementation.
• R&D workflows requiring omission of L-glutamine so that fresh glutamine or stabilized alternatives can be added at the point of use.
• Processes where HEPES is not desired in the base medium and bicarbonate buffering under 5–10% CO2 incubation is preferred.
• Scientific and basic research programs that need a procurement-ready medium with defined bottle size, shelf life, endotoxin limit, and storage requirements.

Store CCM-SF-0064 at 2–8°C and protect the bottle from light to preserve the phenol red indicator and overall formulation integrity. Do not freeze unless your internal validation has confirmed acceptability for the specific workflow. The stated shelf life is 12 months when the product is stored unopened under recommended conditions. Use aseptic technique after opening, minimize repeated warming cycles, and pre-warm only the volume needed for immediate culture work. Product is shipped on blue ice, and receiving teams should inspect the bottle for seal integrity, clarity, and expected color before release into inventory.

CellCultureMedia supplies CCM-SF-0064 through ISO-aligned manufacturing and quality controls appropriate for research-use cell culture materials. Lot documentation can be requested through the quality documentation team and typically covers identity, appearance, pH, sterility, endotoxin, and other release attributes such as osmolality where included in the lot record. The product specification includes sterile filtration, pH 7.0–7.4, endotoxin ≤1 EU/mL, 12-month shelf life, 2–8°C storage, and blue-ice shipment. For standing orders, multi-bottle purchases, or supply planning, submit requirements through request a quote; free worldwide shipping is available for standard online orders.