Human Diploid Cell Serum-Free Medium

CCM-SF-0045 is designed for human diploid cell culture workflows that require a defined liquid medium with visible pH indication and a ready-to-use amino acid supplement profile. The formulation includes phenol red, D-glucose, and 4 mM L-glutamine. Source specifications identify the operating condition as 5% FBS where that supplementation strategy is required by the user, while the medium classification is animal-origin-free for the supplied liquid formulation. For related formulations, see the serum-free media category.

Key release specifications include pH 6.7–7.3 and osmolality 260–320 mOsmol/kg, supporting routine process comparison, lot qualification, and incoming material checks. The medium is sterile-filtered, with endotoxin specified at ≤3 EU/mL and a shelf life of 12 months when stored as directed. The 500 mL bottle size is suitable for R&D scale culture, scale-down model work, and controlled evaluation before larger-volume procurement.

• Expansion and maintenance studies using human diploid cells in 37 °C, 5–10% CO2 incubator conditions.
• Process development for viral antigen, vector, or biopharmaceutical production platforms where HDC substrates are evaluated.
• Medium screening, lot comparability, and raw-material qualification programs requiring documented pH, osmolality, sterility, endotoxin, and shelf-life attributes.
• Reduced-serum operating models where the user’s protocol specifies 5% FBS addition while retaining a defined liquid medium base.
• Academic, biotech, and contract research workflows that need a procurement-ready 500 mL format with quotation support through CellCultureMedia request quote.

Store CCM-SF-0045 at 2–8 °C protected from light. Do not expose the bottle to repeated warming cycles longer than necessary for aseptic use. Mix gently before dispensing, avoid foaming, and handle under validated aseptic technique after opening. The product ships on blue ice to help maintain refrigerated transit conditions. Shelf life is 12 months from the applicable manufacturing or release date when the product remains unopened and is stored under the specified refrigerated, light-protected conditions.

CellCultureMedia supplies this medium for research and bioprocessing use with quality controls aligned to ISO-style manufacturing and release practices. Lot-specific documentation can include Certificate of Analysis data on request, including sterility, endotoxin, pH, osmolality, and appearance where applicable. Procurement teams may request batch documentation, pack-size confirmation, and shipping planning through the CellCultureMedia team. Learn more about documentation expectations on the quality and compliance page. Free worldwide shipping is available for qualified orders, with pricing for the 500 mL unit listed from $319.44.