Human Diploid Cell ADCF Powder Medium

CCM-SF-0046 is a dry powder cell culture medium designed for human diploid cells in animal-origin-free workflows. The formulation includes HEPES buffering, D-glucose, and 4 mM L-glutamine, and it is supplied without phenol red to reduce colorimetric interference in optical measurements and downstream analytical readouts. The product is categorized within serum-free media, while the source specification also notes use with 5% FBS where a customer process requires serum supplementation. This makes the medium suitable for laboratories that are qualifying either defined or reduced-serum operating conditions under controlled documentation.

The powder format supports 50 L final preparation and is useful where shipping weight, storage efficiency, and batch-to-batch preparation control are important procurement factors. Key release specifications include pH 5.7–6.3 in the supplied medium system, osmolality 210–270 mOsmol/kg, and endotoxin not more than 3 EU/mL. Standard culture conditions are 37 °C with 5–10% CO2, subject to site-specific cell adaptation and process settings. The medium is positioned for vaccine and biopharmaceutical process development, cell expansion studies, and scale-up evaluation using human diploid cell substrates.

• Expansion and maintenance studies for human diploid cells under 37 °C, 5–10% CO2 incubation conditions.
• R&D and process-development workflows requiring animal-origin-free medium components and documented endotoxin, pH, and osmolality specifications.
• Evaluation of phenol red-free culture conditions for assays where optical density, fluorescence, or color-based readouts may be affected by indicator dye.
• Pilot-scale medium preparation where a 50 L powder pack helps reduce cold-chain volume versus equivalent ready-to-use liquid medium.
• Biopharmaceutical production-support studies, including cell growth, process comparability, raw-material qualification, and upstream parameter screening.
• Serum-reduction or serum-supplemented workflows, where the listed source condition of 5% FBS is incorporated according to an established internal protocol.

Store unopened powder at 2–8 °C protected from light. The stated shelf life is 24 months when stored under the recommended conditions and handled using appropriate clean laboratory practices. Shipments are arranged on blue ice to maintain the specified cold-chain profile during transit. After receipt, inspect the container, verify labeling against the purchase order, and place the material into controlled refrigerated storage promptly. During reconstitution, use validated water quality, calibrated weighing and mixing equipment, and site-approved filtration or sterilization procedures as applicable to the final-use process. Because the product contains L-glutamine, avoid unnecessary warming or prolonged hold times after preparation, and define in-house hold limits for prepared medium based on local qualification data.

CellCultureMedia supplies CCM-SF-0046 for professional cell culture, research, and bioprocessing environments that require consistent specification review before purchasing. Manufacturing and release controls are managed within ISO-aligned quality practices, with lot-level documentation available to support supplier qualification, incoming inspection, and change-control files. A Certificate of Analysis can be requested through quality documentation support, including applicable release data such as appearance, endotoxin, pH, osmolality, and sterility-related testing where available for the lot. For budgetary planning, the listed 50 L pack price is $15,080.56, and free worldwide shipping is available for qualified orders. Procurement teams can use the request quote workflow to confirm current availability, delivery window, bulk pricing, documentation requirements, and any site-specific shipping instructions before order placement.