Glucose-Free CHO Feed Supplement Powder

CCM-SF-0028 is a concentrated dry powder feed supplement for CHO-based fed-batch and process development workflows. It is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 systems and is used without serum addition. The product is formulated for biopharmaceutical and vaccine-platform cell culture operations where feed timing, glucose setpoints, and glutamine strategy are controlled by the process engineer. The powder format supports controlled reconstitution into a 10 L working preparation and is suitable for laboratories that qualify raw materials by composition exclusions, storage profile, and lot documentation.

This feed is animal-origin-free and is supplied without phenol red, D-glucose, or L-glutamine. The absence of D-glucose allows users to decouple carbon-source addition from amino acid, vitamin, and nutrient feeding. The absence of L-glutamine supports processes that use alternative glutamine sources, controlled glutamine addition, or glutamine-free operating strategies. The recommended culture environment is 37 °C with 5-10% CO2, consistent with standard CHO suspension culture conditions. Endotoxin is specified at ≤20 EU/mL. Related products can be reviewed in the serum-free media category.

• Fed-batch CHO process development where glucose concentration is monitored and adjusted independently from the main nutrient feed.
• Serum-free CHO K1, CHO-S, CHO-GS, and CHO DG44 culture workflows used for clone screening, scale-down modeling, and upstream parameter optimization.
• Bioreactor and shake-flask studies requiring an animal-origin-free feed with defined exclusions for phenol red, D-glucose, and L-glutamine.
• Biopharmaceutical production development, including recombinant protein and vaccine-platform cell culture processes, where raw-material consistency and documented release criteria are required.
• Procurement programs that need a 10 L powder-format feed supplement with lot traceability, defined storage conditions, and qualification data available before purchase.

Store CCM-SF-0028 at 2-8 °C and protect it from light. The stated shelf life is 24 months when the unopened product is stored under the specified conditions. Shipments are arranged on blue ice to help maintain temperature exposure during transit, and free worldwide shipping is included for qualified orders. After receipt, inspect packaging, record lot information, and move the product promptly to refrigerated storage. Reconstitution, filtration strategy, hold time, and in-process bioburden controls should be defined by the user’s internal media-preparation procedure. For ordering questions or pack-size planning, use the request quote form.

CellCultureMedia supplies CCM-SF-0028 for research, development, and bioprocessing use with procurement-oriented documentation support. Manufacturing and release controls are managed under ISO-aligned quality practices, with lot traceability and Certificate of Analysis availability on request. The CoA can support incoming quality review by confirming product identity, lot number, endotoxin specification, and other release information provided for the batch. If your team requires qualification documents, change-notification expectations, or raw-material review before ordering, contact CellCultureMedia through the quality documentation page. Bulk and repeat-order pricing can be quoted for platform studies, pilot campaigns, and multi-site procurement programs.