DF-1 Serum-Free Powder Medium

CCM-SF-0048 is a serum-free, animal-origin-free powdered medium specified for chicken embryo dermal fibroblast DF-1 cell culture. It is supplied for preparation of 10 L of finished medium and is intended for laboratories and production groups that require a defined alternative to serum-containing formulations. The powder includes D-glucose for carbohydrate supply, 0.5 mM L-glutamine, and 1 mM L-alanyl-glutamine to support nitrogen metabolism and improved glutamine stability during handling. The formulation is phenol red-free, making it suitable for users who monitor cultures using fluorescence, absorbance, or other readouts where indicator dye may affect background signal.

This product belongs to the serum-free media portfolio and is aligned with DF-1 expansion, adaptation, and process-development workflows. Recommended culture conditions are 37 °C with 5–10% CO2. The listed shelf life is 24 months when stored as directed, and the endotoxin specification is ≤3 EU/mL. pH and osmolality values are not assigned in the public specification for this powder; if these parameters are required for an internal release protocol, request lot-specific documentation during quotation.

• Routine culture and expansion of DF-1 chicken embryo fibroblast cells in a serum-free environment, including adaptation studies from serum-containing systems where a defined medium is required.
• Avian cell process development for vaccine and biopharmaceutical research workflows, including upstream screening, seed-train planning, scale-down model evaluation, and medium comparison studies.
• Phenol red-free cell culture assays where reduced color background is important for imaging, fluorescence, absorbance, metabolic monitoring, or automated plate-reader workflows.
• Animal-origin-free media qualification programs where procurement, quality, and process engineering teams need a documented formulation class, controlled storage requirements, and consistent powder packaging for inventory planning.
• Powder-format medium preparation for organizations that prefer lower shipping weight, longer inventory flexibility, and site-controlled reconstitution. For volume forecasts, standing orders, or alternate pack planning, use the request quote workflow and include the target number of 10 L equivalents.

Store unopened powder at 2–8 °C and protect from light. The specified shelf life is 24 months under these conditions. Shipments are arranged on Blue Ice to maintain controlled transit conditions; inspect the package on receipt and move the material promptly to refrigerated storage. During preparation, use validated site procedures for weighing, hydration, mixing, pH adjustment if required by the process, and final filtration or handling steps defined by the end user’s quality system. Because the medium contains glutamine and L-alanyl-glutamine, avoid unnecessary warming cycles and keep prepared medium exposure within locally validated hold times.

CellCultureMedia supplies CCM-SF-0048 for research and bioprocessing use under ISO-aligned manufacturing and supplier-control expectations. Lot documentation is available on request and can support incoming inspection, change-control review, and raw-material qualification. The stated endotoxin limit is ≤3 EU/mL; sterility-related release information, pH, osmolality, and other lot-specific attributes should be confirmed through the Certificate of Analysis or supporting quality documents when required by your internal specification. Visit quality documentation for general documentation practices, or request a quotation for current lot availability, lead time, and bulk pricing. Qualified orders are supported with free worldwide shipping, helping procurement teams compare landed cost across international receiving sites.