DF-1 Chicken Embryo Fibroblast Serum-Free Medium

DF-1 Chicken Embryo Fibroblast Serum-Free Medium is a ready-to-use liquid formulation for laboratories and process teams culturing chicken embryo dermal fibroblast DF-1 cells without serum supplementation. The medium is classified as animal-origin-free and serum-free, helping teams reduce undefined raw-material inputs during research scale-up, platform comparison, and controlled cell-culture workflows. It is supplied under CCM-SF-0047 in a 500 mL format for convenient qualification, routine bench use, and small-batch process development.

The formulation includes phenol red for visual pH indication, D-glucose as a carbohydrate source, 0.5 mM L-glutamine, and L-alanyl-L-glutamine for improved glutamine stability during culture. Recommended culture conditions are 37 °C with 5-10% CO2. Key release specifications include sterile-filtered liquid form, pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin ≤3 EU/mL, and a 12-month shelf life when stored as directed. For related formats, visit our serum-free media category.

• Serum-free culture of DF-1 chicken embryo dermal fibroblast cells in R&D and process-development environments.
• Evaluation of animal-origin-free media conditions where reduced raw-material variability is required.
• Adherent cell expansion studies using phenol-red-containing medium for routine monitoring of culture status.
• Biopharmaceutical and viral production process research where DF-1 cells are part of an upstream workflow.
• Comparability studies between serum-containing and serum-free culture systems during media selection.
• Procurement-controlled supply for academic, biotech, and bioprocess laboratories requiring documented specifications, defined storage conditions, and repeatable ordering under a CCM catalog identity.

Store CCM-SF-0047 at 2-8 °C protected from light. Do not use beyond the stated 12-month shelf life or after visible signs of contamination, precipitation beyond normal formulation behavior, or container compromise. The product ships on blue ice to maintain refrigerated transit conditions; upon receipt, verify bottle integrity, label identity, lot number, and temperature condition before placing into controlled cold storage. Use aseptic technique when opening and dispensing. For process continuity, avoid repeated warming cycles and aliquot only in sterile, validated containers when required by internal procedures.

CellCultureMedia supplies CCM-SF-0047 for research and bioprocessing workflows that require clear specifications, lot traceability, and purchasing consistency. Manufacturing and release controls are ISO-aligned, with sterile filtration, endotoxin testing, pH verification, and osmolality testing included as applicable to the finished liquid medium. A Certificate of Analysis is available on request, and procurement teams may contact us through request a quote for volume pricing, lead-time confirmation, and recurring supply planning. Quality documentation support is available through our quality team. Free worldwide shipping is included for eligible standard orders, including the 500 mL bottle size listed at $319.44.