CHO Serum-Free Medium Without Phenol Red or Glutamine

CCM-SF-0016 is a ready-to-use liquid CHO serum-free medium designed for 37°C culture with 5-10% CO2 in research, development, and bioprocess environments. It supports CHO K1, CHO-S, CHO-GS, and CHO DG44 workflows where consistent medium chemistry, reduced animal-origin inputs, and defined supplementation are procurement and process requirements. The product is supplied in a 500 mL format and is positioned within CellCultureMedia's serum-free media portfolio for teams standardizing CHO culture across screening, scale-up, and production-support studies.

The formulation contains D-glucose and 2.2 g/L sodium bicarbonate, and it is intentionally manufactured without serum, phenol red, L-glutamine, or HT additives. Excluding phenol red can be useful for optical or assay-sensitive workflows, while omission of L-glutamine allows users to select the preferred glutamine source, concentration, or stabilized substitute. The absence of HT additives is relevant when teams need to manage nucleotide-pathway supplementation independently. Release specifications include pH 7.0-7.4, osmolality of 270-340 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterility by filtration. These specifications help process engineers compare lots, set incoming acceptance criteria, and align media purchasing with documented cell culture operating ranges.

• Serum-free suspension culture of CHO K1, CHO-S, CHO-GS, and CHO DG44 cells under 37°C, 5-10% CO2 conditions.
• Development of animal-origin-free CHO processes for recombinant protein expression, clone evaluation, feed strategy studies, and upstream process characterization.
• Media screening programs where phenol red-free conditions are preferred for colorimetric, fluorescence, imaging, or analytical readouts.
• Workflows requiring user-defined L-glutamine addition, including comparison of fresh L-glutamine, stabilized glutamine substitutes, or glutamine-free process designs.
• CHO culture studies that require independent management of HT supplementation rather than a pre-supplemented medium.
• Biopharmaceutical and academic R&D programs seeking a specified 500 mL serum-free medium with documented pH, osmolality, endotoxin, sterility, and shelf-life attributes.

Store CCM-SF-0016 at 2-8°C protected from light. Do not store at room temperature for extended periods, and avoid repeated warming cycles where possible. The product ships on blue ice to help maintain refrigerated conditions in transit; inspect bottles on receipt and place them promptly at 2-8°C. The stated shelf life is 12 months when stored as recommended and handled using aseptic technique. Before use, equilibrate only the required volume to culture temperature, mix gently to ensure uniformity, and avoid vigorous shaking that may introduce foam. Because the medium does not contain L-glutamine or HT additives, add any user-selected supplements aseptically and document final concentrations in the batch or experiment record. For price confirmation, temperature-lane planning, or standing-order setup, teams can use the request quote channel.

CellCultureMedia supplies CCM-SF-0016 for controlled cell culture procurement where lot traceability, defined release testing, and documentation access are important. Manufacturing and quality controls are ISO-aligned, and the medium is filter-sterilized with release specifications for pH, osmolality, endotoxin, and sterility. A Certificate of Analysis is available on request, and procurement teams may request lot-specific documentation before ordering or at shipment. CellCultureMedia can support single-bottle evaluation orders, recurring supply, and bulk purchasing for multi-site R&D or process-development groups. Visit quality documentation for an overview of documentation practices, or contact the sales team for shipping lane, lead-time, and quotation support. Free worldwide shipping may be applied to eligible orders, including the 500 mL CCM-SF-0016 format priced from $319.44.