CHO Serum-Free Animal Component-Free Medium

This CHO serum-free medium is a ready-to-use liquid formulation designed for serum-free and animal component-free cell culture. It supports controlled development work where researchers need a phenol red-free background for optical or analytical readouts, and where L-glutamine and sodium bicarbonate are managed separately according to the process design. D-glucose is included. For related formulations and scale-up options, review the serum-free media category.

Key release specifications include sterile-filtered liquid format, pH 7.0–7.4, osmolality 270–340 mOsmol/kg, and endotoxin not more than 3 EU/mL. The medium is intended for incubation at 37 °C with 5–10% CO2, subject to the user’s vessel, buffering strategy, and supplementation plan. The formulation is suitable for evaluation in biopharmaceutical expression and vaccine process development environments where serum-free, animal component-free raw materials are specified.

• Serum-free culture development for CHO K1, CHO-S, CHO-GS, and CHO DG44 cell lines.
• R&D screening studies where phenol red may interfere with absorbance, fluorescence, or colorimetric analytical methods.
• Process engineering studies that require external control or separate addition of L-glutamine and sodium bicarbonate.
• Small-volume feasibility testing before transition to larger media packs or process-specific custom specifications.
• Biopharmaceutical and vaccine-related cell culture workflows that require animal component-free sourcing and defined lot documentation.

The 100 mL pack size is appropriate for qualification work, compatibility checks, and early experimental design. For larger purchasing quantities or program-specific stocking, use the request quote page to confirm current availability, price breaks, and logistics options.

Store CCM-SF-0014 at 2–8 °C protected from light. Do not expose the bottle to prolonged room-temperature holding, direct light, or repeated warming cycles. The specified shelf life is 12 months when stored unopened under recommended conditions. Product is shipped on blue ice to help maintain refrigerated transit conditions. On receipt, inspect the container, confirm that the liquid is clear and free from visible contamination, record the lot number, and return the product to refrigerated storage promptly. Because the supplied formulation does not contain L-glutamine or sodium bicarbonate, add any process-specific supplements using validated aseptic technique and qualified raw materials.

CellCultureMedia supplies CCM-SF-0014 for research, development, and bioprocessing use with procurement documentation suitable for supplier qualification files. Manufacturing and release controls are ISO-aligned, and lot-specific Certificate of Analysis documentation is available on request, including pH, osmolality, sterility, endotoxin, and shelf-life data where applicable. Additional quality information can be reviewed through the quality resources page. The listed 100 mL price is USD 319.44, and free worldwide shipping is available for standard orders where courier service and import conditions permit.