CHO Serum-Free ADCF Medium, Phenol Red-Free

CCM-SF-0018 is a sterile-filtered CHO serum-free medium designed for users who need a chemically defined-style, animal-derived-component-free liquid medium for upstream cell culture evaluation. It is suitable for procurement teams standardizing supply of serum-free media and for process groups comparing media performance across CHO K1, CHO-S, CHO-GS, and CHO DG44 platforms. The phenol red-free format supports optical or fluorescence-based analytical workflows where indicator dye interference is undesirable.

The product is supplied as a liquid medium with D-glucose added and without L-glutamine. This configuration gives users flexibility to add a preferred glutamine source or stable dipeptide supplement according to internal protocols, feed strategy, and cell line requirements. Core release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterile-filtered status. Recommended culture conditions are 37 °C with 5-10% CO2, aligned with common CHO suspension culture practice. The 100 mL pack size is appropriate for screening, seed-train evaluation, small-scale shake flask work, and qualification before larger purchasing commitments.

• Serum-free expansion and maintenance studies for CHO K1, CHO-S, CHO-GS, and CHO DG44 cell platforms used in recombinant protein expression and upstream process development.
• Animal-derived-component-free media screening where supply-chain traceability, reduced lot-to-lot variability, and simplified raw material assessment are procurement priorities.
• Phenol red-free culture workflows requiring lower background for absorbance, imaging, fluorescence, or metabolite monitoring assays.
• Small-volume medium evaluation before scale-up into shake flasks, spinner vessels, benchtop bioreactors, or fed-batch development programs.
• Glutamine-strategy optimization, because the base medium is supplied without L-glutamine and can be supplemented under user-controlled conditions.
• Academic, biotech, and biopharma R&D groups seeking defined CHO culture inputs with documented sterility, endotoxin, pH, osmolality, shelf-life, and shipping parameters.

Store CCM-SF-0018 at 2-8 °C protected from light. Do not freeze unless an internal stability assessment supports that practice, as freeze-thaw exposure can affect physical appearance and performance of liquid media. The stated shelf life is 12 months when stored unopened under recommended conditions. Use aseptic technique after opening, minimize repeated warming cycles, and equilibrate only the volume needed for current work. Because the medium is L-glutamine-free, add any required glutamine, stable glutamine substitute, or cell line-specific supplement immediately before use or according to a validated internal procedure. Product is shipped on blue ice to support temperature-controlled transit.

CellCultureMedia supplies CCM-SF-0018 for research and bioprocessing customers who require specification-driven purchasing. Manufacturing and release controls are ISO-aligned, and lot documentation can be requested through the quality documentation channel. A Certificate of Analysis is available on request and may include lot-specific confirmation of key attributes such as pH, osmolality, sterility, endotoxin, appearance, shelf life, and storage requirements. Free worldwide shipping is included for this item, with blue-ice shipment as the standard condition. For standing orders, larger pack planning, or multi-site sourcing, submit requirements through request a quote so our team can confirm availability, shipping lane options, and volume pricing.