CHO Feed 3 Supplement, Serum-Free and Animal-Origin-Free

CCM-SF-0029 is a sterile-filtered liquid feed supplement for serum-free CHO culture. The formulation is animal-origin-free and is supplied without phenol red and without L-glutamine, supporting defined media strategies where users manage glutamine or alternative nitrogen sources separately. D-glucose is included to support fed-batch energy input. The product is intended for use with compatible serum-free media in research, process development, and bioprocessing environments.

Key release specifications include pH 6.8 to 8.0, endotoxin not more than 20 EU/mL, and sterile-filtered liquid format. The recommended culture environment for associated CHO workflows is 37 °C with 5-10% CO2, subject to the user’s cell line and platform settings. The absence of phenol red can reduce optical background in color-sensitive analytical methods, while the absence of L-glutamine provides flexibility for users who control glutamine addition, substitution, or feed timing as part of a validated internal protocol.

• Fed-batch supplementation for CHO K1, CHO-S, CHO-GS, and CHO DG44 cultures in serum-free production and development platforms.
• Evaluation of animal-origin-free feed strategies for recombinant protein and biologics process development, including scale-down models and media-feed screening.
• Defined feeding workflows where D-glucose input is required and L-glutamine is intentionally managed outside the feed supplement.
• Color-sensitive culture monitoring or assay workflows where phenol red is not desired in the feed stream.
• Procurement programs requiring a liquid, sterile-filtered, serum-free feed supplement with documented pH, endotoxin, shelf-life, storage, and shipping parameters.

Store CCM-SF-0029 at 2-8 °C and protect the container from light. Do not use after the assigned expiry date; the stated shelf life is 12 months when stored as recommended and kept unopened. Shipments are handled on blue ice to maintain refrigerated transit conditions. On receipt, inspect the bottle and closure, confirm that the product remains clear and free from visible contamination, and transfer promptly to controlled refrigerated storage. Use aseptic technique during handling, and avoid repeated warming cycles or extended exposure at room temperature. If a process requires pre-use equilibration, limit warming time and mix gently without foaming before addition to culture.

CellCultureMedia supplies CCM-SF-0029 for professional cell culture procurement programs that require traceable specifications and consistent documentation. Manufacturing and release controls are ISO-aligned, and each lot is evaluated against defined physical, chemical, and microbiological criteria, including sterile filtration, pH range, and endotoxin limit. A Certificate of Analysis is available on request through our quality documentation team, and lot-specific documentation can be matched to your purchase order where required. For standing orders, project forecasts, or multi-site supply, submit a request through request a quote. Free worldwide shipping is available for qualifying orders, with refrigerated blue-ice logistics used for this liquid product.