Chemically Defined 293 Serum-Free Medium

This chemically defined 293 serum-free medium is supplied in liquid form for laboratories and manufacturing groups running suspension HEK293-derived cultures without serum supplementation. The formulation is animal-origin-free and contains a dual glutamine system: 2 mM L-glutamine for immediate nitrogen availability and 2 mM L-alanyl-L-glutamine for improved stability during routine culture use. D-glucose is included as the primary carbohydrate source. The absence of phenol red is useful for workflows where optical readouts, downstream color control, or formulation transparency are important.

Key release specifications include pH 7.0 to 7.4, osmolality 270 to 340 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterility by filtration. The medium is intended for 37 °C culture with 5 to 10% CO2, consistent with common humidified incubator conditions for 293 suspension platforms. The 500 mL pack size supports R&D screening, seed train evaluation, small-batch expression runs, and qualification before scale-up. For standing orders, multi-case planning, or forecast-based supply, procurement teams can request a quote with target delivery windows, destination, and required documentation.

• Serum-free expansion of HEK293-derived suspension cultures used in transient expression, stable clone assessment, and upstream process development.
• Animal-origin-free culture workflows where undefined serum components are not acceptable for reproducibility, documentation, or downstream purification planning.
• Protein, viral vector, and biologics R&D processes requiring a phenol-red-free medium background for analytical compatibility and visual clarity.
• Media comparison studies, feed strategy development, and passage adaptation projects where pH, osmolality, endotoxin, and sterility specifications must be documented.
• Seed train preparation and bench-scale shake flask or controlled vessel evaluation under 37 °C, 5 to 10% CO2 culture conditions.

Store CCM-SF-0058 at 2 to 8 °C protected from light. The specified shelf life is 12 months when the product remains unopened and is stored under recommended conditions. Shipments are dispatched on blue ice to help maintain refrigerated transit conditions; inspect containers on receipt and place them at 2 to 8 °C promptly. Mix gently before use and avoid repeated warming cycles. Use aseptic technique when dispensing, and do not return unused medium from open vessels to the original bottle. End users should qualify hold time after opening according to internal procedures and contamination-control requirements.

CellCultureMedia supplies this medium for research and bioprocessing environments that require procurement traceability, batch documentation, and consistent specification review. Manufacturing and release controls are ISO-aligned, with filtration-based sterility processing and lot-based testing for pH, osmolality, endotoxin, appearance, and other applicable attributes. A Certificate of Analysis is available on request, and additional documentation needs can be reviewed through the quality documentation channel. Free worldwide shipping is available where import rules and cold-chain routing permit; quote responses can include price breaks, carton configuration, and estimated dispatch timing for planned demand.