Serum-Free CHO Medium Powder, 50 L

CCM-SF-0015 is a dry powder serum-free medium designed for CHO cell culture where defined composition, lot-to-lot consistency, and animal-origin-free sourcing are important procurement and process requirements. The product is supplied as a 50 L preparation format for laboratories and development groups that prefer compact inventory, controlled reconstitution, and flexible preparation scheduling. It is intended for use with CHO K1, CHO-S, CHO-GS, and CHO DG44 platforms operated under standard incubator conditions of 37 °C with 5-10% CO2.

The formulation does not require serum addition and is specified as animal-origin-free. D-glucose is included. Phenol red, L-glutamine, and HT supplement are not included, allowing users to manage those variables according to internal process design, cell-line requirements, and supplementation strategy. Reported specifications include osmolality of 270-310 mOsmol/kg, endotoxin ≤3 EU/mL, and pH level of 3.7-4.3 for the supplied product specification. The powder format supports biopharmaceutical and vaccine-production research workflows, including medium preparation for screening, adaptation, seed train studies, and controlled shake-flask or small-bioreactor evaluation.

• Serum-free culture of CHO K1, CHO-S, CHO-GS, and CHO DG44 cells in research and process-development environments.
• Medium platform evaluation for expression studies, clone screening, fed-batch optimization, and suspension culture scale-up.
• Animal-origin-free process studies where removal of serum-derived variability is required for reproducibility and supply-chain control.
• Preparation of phenol-red-free base medium for analytical workflows where color indicator interference is undesirable.
• Custom supplementation studies using separately controlled L-glutamine, HT supplement, feeds, hydrolysates, lipids, or process-specific additives.
• Procurement programs requiring a defined 50 L powder format with clear endotoxin, osmolality, storage, and shelf-life specifications.

Store CCM-SF-0015 at 2-8 °C protected from light. The stated shelf life is 24 months when stored unopened under the recommended conditions. Shipments are handled on blue ice to help maintain temperature control during transit, and free worldwide shipping is available for eligible orders. Reconstitute using validated water quality, mixing equipment, and filtration procedures established by your facility. Because this product is supplied without L-glutamine and HT supplement, add any required supplements only after confirming compatibility with the intended CHO cell line and process conditions.

CellCultureMedia supplies CCM-SF-0015 for research, cell culture, and bioprocessing use with quality controls aligned to ISO-style manufacturing and release documentation practices. A Certificate of Analysis is available on request and may include lot-specific confirmation of appearance, sterility-related release testing, endotoxin limit, osmolality range, pH specification, storage condition, and shelf-life assignment. For audit files, vendor qualification, or multi-site purchasing, contact the team through quality documentation support or submit a purchasing request via request a quote. Pricing for the 50 L format is listed at $15,080.56, with bulk and recurring-order pricing available for qualified accounts.