Reduced-Serum Medium Powder, 100 L

CCM-SF-0067 is a powdered reduced-serum medium format intended for preparation of up to 100 L of working medium for mammalian cell culture. The base formulation is classified as animal-origin-free and animal-derived component-free, while the source specification indicates use in reduced-serum culture with 5% FBS where required by an established cell line protocol. This makes the product suitable for labs and process teams transitioning from higher-serum formulations toward more controlled, lower-serum operating conditions.

The medium supports multiple suspension and adherent mammalian cell types, with a chemistry package designed for routine culture control: phenol red for visual pH indication, D-glucose as a carbon source, L-glutamine for nitrogen and energy metabolism, sodium pyruvate as an additional energy substrate, and HEPES for supplemental buffering. Key release specifications include pH 4.6–5.0, osmolality 230–270 mOsmol/kg, and endotoxin not more than 1 EU/mL. For related media formats, see our serum-free and reduced-serum media category.

• Reduced-serum culture of suspension mammalian cells maintained at 37 °C in 5–10% CO2 incubator environments.
• Adherent mammalian cell culture when the laboratory protocol specifies a lower-serum basal environment with optional 5% FBS supplementation.
• Research-scale media preparation for assay development, cell expansion, transfection support studies, and upstream process evaluation.
• Comparability work where procurement teams need a powder format, defined additive profile, endotoxin specification, and 36-month shelf-life target.
• Academic, biotech, and biopharma R&D workflows requiring consistent lot documentation and scalable order planning through custom quotation support.

Store the unopened powder at 2–8 °C protected from light. The stated shelf life is 36 months when the product is maintained under recommended storage conditions and packaging integrity is preserved. Shipments are handled on blue ice to support temperature control in transit; inspect the container on receipt and move it promptly to refrigerated storage. During reconstitution, use validated water quality, controlled mixing, and sterile filtration or other site-approved processing steps appropriate to the downstream cell culture workflow. Record lot number, preparation date, final volume, and any serum or additive supplementation used in the batch record.

CellCultureMedia supplies CCM-SF-0067 for research and bioprocess development use under ISO-aligned supplier qualification, lot traceability, and documentation practices. A Certificate of Analysis is available on request and can support review of identity, appearance, pH, osmolality, endotoxin, and other lot-specific release data. Procurement teams may request availability, lead time, and volume pricing before purchase, and qualifying orders include free worldwide shipping. For documentation expectations and supplier controls, visit the CellCultureMedia quality page.