Animal-Origin-Free CHO Feed 1 Supplement

This CHO Feed 1 supplement is a serum-free, animal-origin-free liquid feed designed for controlled nutrient addition in mammalian cell culture processes. It is supplied sterile-filtered and is positioned for use with serum-free media systems where defined composition, lot traceability, and compatibility with CHO production cell lines are procurement requirements. The formulation excludes phenol red to reduce interference with colorimetric assays and optical monitoring, and excludes L-glutamine to allow users to manage glutamine or stabilized dipeptide strategies separately.

The product contains D-glucose and is intended as a feed supplement rather than a stand-alone complete growth medium. It is suitable for fed-batch optimization, scale-down model development, productivity screening, and routine process support in research and bioprocessing laboratories. Source specifications define the format as liquid, serum-free, and animal-origin-free, with sterile filtration, endotoxin specification of ≤20 EU/mL, and a 12-month shelf life when stored as directed. pH and osmolality requirements should be evaluated against the user’s base medium and feeding schedule; lot-specific documentation may be requested where available.

• Fed-batch feeding studies for CHO K1, suspension-adapted CHO, glutamine synthetase-selected CHO, and DG44 cell culture platforms.
• Nutrient supplementation in serum-free workflows requiring animal-origin-free raw materials and defined feed components.
• Process development experiments comparing feed timing, feed volume, viable cell density, and product titer in shake flasks, bench bioreactors, or scale-down models.
• Workflows where phenol red-free composition is preferred for optical density, fluorescence, absorbance, or metabolite readouts.
• Manufacturing science, R&D, and academic cell culture programs requiring a documented liquid supplement with sterile-filtered release criteria.

Store CCM-SF-0026 at 2–8 °C and protect from light. Do not use beyond the stated 12-month shelf life, and avoid repeated warming cycles that may affect feed consistency. Bring the required volume to room temperature only for the time needed for aseptic addition, then return the remaining closed container to refrigerated storage. Use standard aseptic technique, confirm container integrity before use, and evaluate the feed within the validated feeding strategy for the selected CHO host, base medium, inoculation density, and CO2 set point of 5–10% at 37 °C.

CellCultureMedia supplies CCM-SF-0026 for research and bioprocessing procurement programs that require consistent documentation and responsive international fulfillment. Manufacturing and release practices are ISO-aligned, with sterility, animal-origin-free status, shelf life, and endotoxin specification supported through controlled documentation. A Certificate of Analysis is available on request through our quality documentation workflow. For price confirmation, standing orders, or multi-unit purchasing beyond the 100 mL pack size, submit a request through request a quote. Free worldwide shipping is available for this product, subject to import requirements and lane-specific handling constraints.