Animal-Origin-Free CD 293 Serum-Free Powder Medium, 50 L

CCM-SF-0056 is a dry powder, animal-origin-free serum-free medium developed for chemically defined HEK293 suspension workflows, including 293F-adapted production and R&D cultures. The formulation is serum-free and does not require serum supplementation, supporting procurement teams that need defined raw materials, reduced lot-to-lot variability, and documentation suitable for bioprocess development files. For adjacent options, see the CellCultureMedia serum-free media category.

The formulation contains D-glucose, 3 mM L-glutamine, and 3 mM L-alanyl-glutamine, combining immediate glutamine availability with a stable glutamine dipeptide source. Phenol red is excluded to reduce color-based assay interference and support processes where optical, fluorescence, or absorbance readouts are monitored. Source specifications for the released material include pH 3.4-4.2, osmolality 250-300 mOsmol/kg, and endotoxin not more than 3 EU/mL. The recommended culture environment is 37 °C with 5-10% CO2, subject to site-specific adaptation, inoculation density, feed strategy, and process validation.

• Serum-free expansion of HEK293-derived suspension cultures used in upstream process development, transfection optimization, and recombinant protein expression studies.
• Animal-origin-free culture systems for biopharmaceutical and vaccine-related research manufacturing workflows where defined media composition and raw-material traceability are important.
• Phenol red-free culture conditions for analytical workflows that require lower background from pH indicators, including plate-based screening, optical monitoring, or downstream assay preparation.
• Scale-up studies from shake flasks to controlled bioreactors, with pH, dissolved gas, osmolality, and viable cell density monitored under the customer’s established process controls.
• Procurement standardization for laboratories requiring a 50 L powder format to reduce storage volume compared with liquid media and to improve batch planning for multi-run campaigns.

Store the unopened powder at 2-8 °C protected from light. The stated shelf life is 24 months when stored under the specified conditions and handled according to good laboratory practice. Shipments are dispatched on blue ice to help maintain temperature control in transit. After receipt, transfer the product promptly to refrigerated storage, avoid repeated warming cycles, and keep the container tightly closed when not in use. Reconstitute using validated water quality, mixing, pH adjustment, and filtration or aseptic controls appropriate to your facility’s process requirements. Confirm final in-use parameters internally before production-scale implementation.

CellCultureMedia supplies CCM-SF-0056 for research and bioprocessing use with procurement support for specifications, lot traceability, and quotation documentation. Manufacturing and release practices are ISO-aligned, with lot-specific Certificate of Analysis availability on request covering applicable release attributes such as endotoxin, osmolality, and pH. Sterility or bioburden documentation can be requested where included for the released lot. Review CellCultureMedia quality information or submit a request for quote for lead time, bulk pricing, shipping lane confirmation, and documentation requirements. Free worldwide shipping is available for eligible orders, subject to destination and logistics feasibility.