BHK-21 Serum-Free Powder Medium

CCM-SF-0044 is a dry powder, serum-free medium designed for BHK-21 cell culture under controlled incubator conditions of 37 °C and 5–10% CO2. The formulation is chemically defined and animal-derived component-free, supporting lot-to-lot control in R&D, seed-train, and process-development environments. It is supplied for 50 L preparation volume and includes D-glucose plus 4 mM L-glutamine, reducing the need for separate glutamine supplementation during routine medium preparation.

The medium is manufactured without phenol red, which can be useful for optical readouts, downstream color-sensitive assays, and processes where indicator dyes are excluded by internal specifications. The product category is serum-free media, with an application fit in biopharmaceutical process development and BHK-based production platforms. Endotoxin specification is ≤3 EU/mL. Source data do not list a fixed final pH or osmolality value; users should verify pH, osmolality, conductivity, and filterability after reconstitution against their internal batch record and cell-culture acceptance criteria.

• BHK-21 suspension or adapted culture workflows requiring serum-free and animal-origin-free medium inputs.
• Research-scale and process-development studies where a powder medium supports larger batch preparation, reduced liquid shipping mass, and controlled reconstitution.
• Indicator-free culture systems where phenol red is not desired for analytical, imaging, or downstream process reasons.
• Biopharmaceutical R&D applications involving BHK-derived cell platforms, including upstream parameter screening, feed strategy evaluation, seed expansion, and comparability studies.
• Procurement standardization programs that require defined composition, documented endotoxin limits, shelf-life planning, and repeatable 50 L preparation units.

Store unopened powder at 2–8 °C and protect from light. The stated shelf life is 24 months when stored as recommended and kept sealed. Shipments are packed on blue ice to maintain controlled transit conditions, with free worldwide shipping available for qualified orders. Reconstitute using validated water quality, mixing time, and filtration procedures according to your site SOP. Because this medium contains L-glutamine, minimize repeated warming and extended hold times after preparation unless stability has been confirmed for your process. Use aseptic technique throughout weighing, dissolution, pH adjustment, and final filtration.

CellCultureMedia supplies CCM-SF-0044 through ISO-aligned manufacturing and release processes suitable for research, development, and bioprocessing procurement controls. Lot documentation can support incoming inspection with product identity, format, shelf life, storage conditions, endotoxin limit, and other applicable release attributes. A Certificate of Analysis is available on request, and additional quality documentation may be coordinated for supplier qualification, material review, or change-control files. For standing orders, safety stock planning, or multi-lot campaigns, contact CellCultureMedia for quotation, documentation needs, and shipping schedule alignment.