Animal Component-Free Vero Serum-Free Medium

CCM-SF-0051 is a chemically defined, animal component-free Vero serum-free medium supplied in liquid format for laboratories and production groups requiring reduced variability versus serum-containing culture systems. The formulation is intended for Vero cell maintenance, expansion, and process development at 37 °C in a humidified 5–10% CO2 environment. It is sterile-filtered and produced for users who need documented specifications for procurement, incoming QC, and batch planning.

The medium contains D-glucose and 1.5 mM L-glutamine and is formulated without phenol red. Absence of phenol red can be useful where colorimetric interference, imaging background, or analytical readout compatibility is a consideration. Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin ≤3 EU/mL, sterile-filtered status, and a 12-month shelf life when stored as directed. For related formats and comparable options, visit our serum-free media category.

• Serum-free Vero cell expansion for research, assay development, and bioprocess optimization programs.
• Animal component-free culture workflows where raw material traceability, simplified documentation, and reduced adventitious material risk are procurement priorities.
• Vero-based viral vector, viral antigen, and biopharmaceutical process development studies requiring consistent liquid medium lots.
• Phenol red-free experimental designs, including optical density checks, fluorescence-based assays, imaging workflows, and analytical methods sensitive to indicator dyes.
• Scale-up evaluations from shake flask or spinner formats into controlled bioreactor studies, subject to user qualification for the selected cell bank and operating parameters.
• Replacement or comparability studies when transitioning from serum-supplemented media to a serum-free platform using defined feed, passage, and adaptation protocols.

Store CCM-SF-0051 at 2–8 °C protected from light. Do not freeze unless a validated internal study supports freeze-thaw use for your application. Before use, equilibrate only the required volume to working temperature and handle aseptically in a qualified biosafety cabinet or clean environment. The product ships on blue ice to maintain refrigerated conditions in transit. Inspect the bottle on receipt for seal integrity, clarity, and evidence of temperature excursion, then record the lot number in your inventory system. Use standard laboratory controls to confirm suitability after prolonged handling or repeated warming events.

CellCultureMedia supplies CCM-SF-0051 for professional cell culture, R&D, and bioprocessing use. Manufacturing and release controls are ISO-aligned and supported by lot-specific documentation. A Certificate of Analysis can be requested for review of sterility, endotoxin, pH, osmolality, shelf-life, and formulation status. For documentation expectations, supplier qualification files, or audit support, contact our team through quality and documentation. Standard size is 500 mL at $319.44, with free worldwide shipping available for qualified orders. For recurring demand, multiple-bottle purchases, or project supply planning, submit a request through request a quote.