ADCF Serum-Free Powder Medium with Phenol Red

CCM-SF-0040 is a dry powder, animal-component-free cell culture medium designed for serum-free or low-serum development workflows in virology research, bioprocess development, and routine propagation of established mammalian cell lines. It is supplied as a 10 L preparation size and includes phenol red for visual pH indication and D-glucose as a carbohydrate source. The formulation excludes L-glutamine and sodium bicarbonate, allowing process engineers to define glutamine strategy, bicarbonate level, and CO2 equilibrium according to site-specific procedures. For related formulations, see the serum-free media category.

Key release specifications include pH 6.0–7.0, osmolality 250–290 mOsmol/kg, and endotoxin not more than 3 EU/mL when prepared and tested according to the applicable lot method. The source specification references a 2% FBS operating condition; users requiring an animal-origin-free process should evaluate supplementation choices accordingly. The powder format supports inventory efficiency, reduced cold-chain volume compared with ready-to-use liquid formats, and flexible batch preparation for R&D or pilot-scale media preparation suites.

• Culture of MDCK, Vero, Marc 145, ST, PK-15, MDBK, HEp-2, and BHK-21 cell lines in research and process development settings.
• Serum-free or low-serum process optimization where defined control of L-glutamine addition and sodium bicarbonate buffering is required.
• Cell expansion and upstream development for vaccine and biopharmaceutical manufacturing research, including platform screening and media comparability work.
• Laboratory preparation of powder medium at controlled osmolality and pH targets for 37 °C incubation with 5–10% CO2.
• Procurement programs that need a documented 10 L powder medium with endotoxin, shelf-life, pH, and osmolality specifications.
• Scale-up planning where teams need consistent media inputs before requesting larger pack sizes through CellCultureMedia quotation support.

Store the unopened powder at 2–8 °C protected from light. The stated shelf life is 24 months when stored under recommended conditions in the original container. Shipments are packed with blue ice unless an alternate logistics arrangement is confirmed. During preparation, use validated purified water and controlled mixing to avoid localized concentration gradients. Because sodium bicarbonate and L-glutamine are not included, add these or alternative buffering and nitrogen-source components only as required by the approved internal protocol. After reconstitution and filtration, users should establish hold time, storage temperature, and in-use acceptance criteria within their own quality system.

CellCultureMedia supplies CCM-SF-0040 for research, development, and bioprocessing use with procurement documentation suitable for institutional purchasing. Manufacturing and release are managed under ISO-aligned quality practices, with lot-specific Certificate of Analysis availability on request. Typical documentation can support review of pH, osmolality, endotoxin, appearance, and applicable sterility status for the released lot. For quality expectations and documentation pathways, visit CellCultureMedia quality resources. Orders may include free worldwide shipping, and bulk pricing is available for qualified recurring demand, standing orders, and multi-site procurement programs.