ADCF Serum-Free CHO Feed 7B Supplement

CCM-SF-0022 is an ADCF serum-free feed supplement designed for CHO culture systems that require a concentrated nutrient addition during production-phase cell culture. The product is supplied as a liquid feed with a high osmolality specification of 1000 to 1400 mOsmol/kg and an alkaline pH specification of 11.0 to 11.4. These attributes are typical of concentrated feed materials and should be considered when defining addition rate, feed timing, working-volume limits, and process-specific pH control strategy.

The supplement is classified as animal-origin-free and serum-free, supporting procurement programs that require defined raw-material inputs and traceable documentation. It is intended for use at 37°C in humidified CO2 incubator or bioreactor environments, with the source culture condition stated as 5% to 10% CO2. The product is relevant to CHO-K1, CHO-S, CHO-GS, and CHO DG44 workflows for recombinant protein expression, clone evaluation, fed-batch optimization, scale-down modeling, and manufacturing-support studies. For projects requiring quotation alignment across media, feeds, and reagents, procurement teams can submit requirements through request a quote.

• Fed-batch CHO process development where a defined, serum-free feed is required to support nutrient replenishment after inoculation and early growth phases.
• CHO-K1, CHO-S, CHO-GS, and CHO DG44 culture platforms used for research expression, screening, cell line evaluation, and upstream process characterization.
• Biopharmaceutical and vaccine-related cell culture manufacturing support where animal-derived component-free raw materials are preferred for supplier qualification and process consistency.
• Shake flask, bench-scale bioreactor, and scale-down model studies that require a consistent liquid feed input with known pH and osmolality specifications.
• Procurement standardization programs where a 50 L feed format helps reduce ordering complexity for repeated development or pilot-scale runs.

Store CCM-SF-0022 at 2°C to 8°C and protect the container from light. Do not use the supplement beyond the labeled expiration date; the stated shelf life is 12 months when stored under recommended conditions. The material is shipped on blue ice to maintain refrigerated transit conditions and should be transferred to controlled cold storage promptly upon receipt. Before use, inspect the container and liquid for integrity and visible contamination. Mix gently if needed, using aseptic technique, and avoid repeated warming cycles or prolonged exposure at room temperature. Because the product has a high pH and concentrated osmolality, addition parameters should be verified in the user’s own medium, vessel, and feed schedule.

Each lot is manufactured under ISO-aligned quality practices and sterile-filtered for cell culture use. Key release specifications include sterile-filtered status, endotoxin not more than 20 EU/mL, pH 11.0 to 11.4, and osmolality 1000 to 1400 mOsmol/kg. A lot-specific Certificate of Analysis is available on request to support raw-material review, receiving inspection, and internal documentation packages. CellCultureMedia can also support recurring supply, bulk pricing, and shipment coordination for multi-site teams. For documentation expectations, supplier review, and quality-system information, visit the quality page or request the applicable lot records with your order.