ADCF CHO Feed Supplement Powder

CCM-SF-0024 is a concentrated powder feed supplement for serum-free CHO cell culture workflows. The formulation is animal-origin-free and supplied without phenol red or L-glutamine, allowing process teams to manage indicator background, glutamine strategy, and feed timing according to the established production platform. D-glucose is included as a defined energy source, making the product suitable for fed-batch processes where nutrient addition is separated from the base growth medium.

The supplement is designed for CHO K1, CHO-S, CHO-GS, and CHO DG44 cultures maintained at 37 ℃ with 5-10% CO2. Key release specifications include pH 4.1-4.7, osmolality 1200-1600 mOsmol/kg, and endotoxin ≤20 EU/mL. The high osmolality profile is consistent with concentrated feed use and should be considered when defining addition volumes, feed schedule, and final culture osmolality. Procurement teams can reference the CCM-SF-0024 identifier for ordering, documentation, and lot traceability.

• Fed-batch CHO process development in serum-free and animal-origin-free media systems.
• Nutrient supplementation for CHO K1, CHO-S, CHO-GS, and CHO DG44 cell culture platforms.
• R&D and scale-up workflows supporting recombinant protein, antibody, vaccine antigen, and biopharmaceutical manufacturing programs.
• Processes requiring feed components without phenol red and without L-glutamine, enabling user-controlled indicator and glutamine management.
• Comparability studies where pH, osmolality, endotoxin level, shelf life, and formulation constraints must be documented for purchasing and engineering review.

Store the powder at 2-8 ℃ protected from light. Shelf life is 24 months when the unopened product is held under the specified conditions. The product ships on blue ice to support temperature-controlled transit, and free worldwide shipping is included for standard orders. After reconstitution, handle using validated aseptic technique, apply sterile filtration or closed-system processing according to site procedures, and record preparation date, lot number, dilution, and in-use hold conditions in the batch record.

CellCultureMedia supplies CCM-SF-0024 for research, development, and bioprocess use under ISO-aligned quality practices. Lot documentation can be requested to support incoming inspection, including Certificate of Analysis details for pH, osmolality, endotoxin, and other available release attributes. For documentation expectations, visit quality and documentation. To confirm batch availability, destination shipping, or volume pricing for multi-lot procurement, use the request quote workflow.