ADCF CHO Feed 1 Supplement Powder

CCM-SF-0023 is a dry powder feed supplement for CHO cell culture programs requiring a serum-free and animal-component-free input. The product is supplied as a 10 L pack size and is intended for preparation as a feed stream in chemically defined or serum-free process development. The formulation contains D-glucose and excludes phenol red and L-glutamine, giving process teams flexibility to manage pH indicators and glutamine strategy separately according to their platform requirements.

This product is part of the CellCultureMedia serum-free media portfolio and is specified for use with CHO K1, CHO-S, CHO-GS, and CHO DG44 systems. It supports controlled culture conditions of 37°C and 5–10% CO2, aligning with common suspension CHO workflows used in recombinant protein, antibody, vaccine process development, and other biopharmaceutical production research. Source specifications list endotoxin at ≤20 EU/mL and a shelf life of 24 months when stored as directed. pH and osmolality should be confirmed after reconstitution and any site-specific supplementation, because final values can depend on preparation procedure, water quality, and feed concentration.

• Fed-batch nutrient supplementation for CHO K1, CHO-S, CHO-GS, and CHO DG44 cultures in shake flask, bench bioreactor, or scale-up development settings.
• Serum-free and animal-component-free workflows where procurement teams require defined inputs and reduced animal-origin risk in upstream operations.
• Process engineering studies comparing feed timing, feed volume, glucose supply, and glutamine management without introducing phenol red or pre-added L-glutamine.
• R&D and manufacturing-support studies for recombinant protein, antibody, and vaccine-related bioprocessing where CHO host platforms are used under controlled CO2 incubation.
• Media optimization projects requiring a powder format for inventory efficiency, long shelf life, and site-controlled reconstitution. For order sizing, documentation needs, or lot reservation, use the request quote workflow.

Store unopened powder at 2–8°C protected from light. The listed shelf life is 24 months when the product remains sealed and is maintained under the recommended conditions. The product ships on blue ice to support temperature control during transit. After receipt, verify the package condition, transfer promptly to refrigerated storage, and record the lot number for traceability. Reconstitute using validated site procedures and qualified water, then mix until fully dissolved before any filtration or addition to culture. Because the formulation is supplied without L-glutamine, add glutamine or an alternative nitrogen source only if required by the receiving process. Prepared feed should be handled using aseptic technique and used according to internal hold-time, sterility, and compatibility studies.

CellCultureMedia supplies CCM-SF-0023 for research and bioprocessing use with procurement documentation suitable for institutional purchasing and supplier qualification. Manufacturing and release practices are ISO-aligned, and a lot-specific Certificate of Analysis can be requested to confirm identity, appearance, endotoxin specification, and other release attributes available for the batch. Additional quality support is available through the quality documentation channel for buyers who need traceability information before purchase approval. The current listed price for the 10 L size is $7,238.89, with free worldwide shipping included where service is available. For standing orders, bulk requirements, or synchronized deliveries across multiple sites, CellCultureMedia can quote scheduled supply options under CCM-SF-0023.