293 Transient Expression Feed Kit

This 293 transient expression feed kit is a liquid, serum-free supplement set for process development and production-scale research using suspension-adapted 293 cell platforms. It is positioned within the serum-free media category and is suitable for organizations standardizing animal-origin-free raw materials in plasmid transfection, recombinant protein expression, and related biomanufacturing research workflows. The kit is intended to be used with 293F and comparable high-density 293 derivatives where defined feeding is required after transfection.

The product is supplied as 1 set under CCM-SF-0031. The source specification identifies the form as liquid, the serum requirement as none, and the classification as animal-origin-free. Recommended culture conditions are 37°C with 5–10% CO2, consistent with common humidified incubator operation for suspension 293 cells. The endotoxin specification is ≤20 EU/mL, and the labeled shelf life is 12 months when stored as directed. Where pH, osmolality, sterility, or additional lot-release values are needed for internal batch records, procurement teams may request current lot documentation before ordering.

• Fed-batch support for transient expression in 293F and compatible suspension 293 culture systems.
• R&D-scale and pilot-scale expression of recombinant proteins, viral vector research materials, and analytical reference materials used in bioprocess development.
• Serum-free workflow standardization where animal-origin-free inputs and controlled supplementation are required.
• Process engineering studies comparing feed timing, feed volume, viable cell density, expression window, and harvest timing under 37°C, 5–10% CO2 conditions.
• Biopharmaceutical research programs requiring a defined liquid feed kit with endotoxin specification, 12-month shelf life, and blue-ice logistics.

Store CCM-SF-0031 at 2–8°C protected from light. Do not leave the kit at room temperature for extended periods during routine use, and return components to refrigerated storage promptly after aliquoting or feeding. The product ships on blue ice to maintain refrigerated transit conditions. Before use, equilibrate only the required volume as appropriate for the validated laboratory procedure, mix gently to ensure homogeneity, and avoid repeated warming cycles. The stated shelf life is 12 months from manufacture or as indicated on the product label and lot documentation.

CellCultureMedia supplies CCM-SF-0031 for research and bioprocessing customers that require clear specifications, consistent packaging, and documentation suitable for vendor qualification. Manufacturing and release controls are managed under ISO-aligned quality practices, with lot-level documentation available through the quality documentation process. A Certificate of Analysis can be requested to confirm applicable release attributes, including endotoxin specification and shelf-life information. For standing orders, multi-site supply, or larger project quantities, use the request quote workflow to confirm price, lead time, and free worldwide shipping options.