Williams E Medium, No Phenol Red, Glutamine or HEPES

CCM-CM-0034 is a classical Williams E formulation supplied as a ready-to-use liquid medium for scientific and basic research. The formulation is specified with 2.0 g/L D-glucose, 50 mg/L glycine, and 25 mg/L sodium pyruvate. It is manufactured without phenol red, L-glutamine, and HEPES, giving process engineers and R&D teams a defined starting point when indicator-free culture conditions, separate glutamine addition, or custom buffering strategies are required. For related formulations, see our classical media portfolio.

Key release specifications support procurement review and incoming quality checks: sterile-filtered liquid form, endotoxin not more than 1 EU/mL, pH 7.0–7.6, and osmolality 305–335 mOsmol/kg. The medium is suited to primary liver cell culture models and other cell systems where the Williams E nutrient profile is required. The absence of phenol red can be useful for optical, fluorescence, or hormone-sensitive assay formats where background signal or indicator chemistry must be minimized.

• Primary liver cell culture workflows requiring Williams E chemistry with controlled user addition of L-glutamine.
• Indicator-free culture conditions where phenol red is not desired for imaging, absorbance, or fluorescence readouts.
• Research-scale medium optimization studies comparing HEPES-free buffering against application-specific supplementation plans.
• Routine maintenance, plating, or experimental culture of compatible liver-derived and metabolically active cell models.
• Procurement programs that require documented pH, osmolality, sterility, endotoxin, storage, and shelf-life specifications for lot release review.

Store CCM-CM-0034 at 2–8 °C protected from light. The product has a 12-month shelf life when kept unopened under recommended refrigerated conditions. Shipments are packed with blue ice packs to maintain cold-chain suitability during transit. Before use, equilibrate only the required volume under aseptic conditions and avoid repeated warming of the full bottle. Because L-glutamine is not included, users should add validated supplements, if required, according to their internal protocol and compatibility assessments.

CellCultureMedia supplies CCM-CM-0034 for research and bioprocess development environments that require consistent documentation and purchasing clarity. Manufacturing and release controls are ISO-aligned, with sterile filtration and lot-specific testing for pH, osmolality, and endotoxin. A Certificate of Analysis is available on request through our quality documentation channel. For standing orders, project-volume demand, or multi-site purchasing, submit requirements through request a quote; free worldwide shipping can be included with eligible quotes.