High-Glucose DMEM with L-Glutamine and Pyruvate

High-glucose DMEM is a widely used liquid medium for routine expansion, maintenance, and experimental culture of adherent mammalian cells. CCM-CM-0004 is positioned within our classical media range and is intended for laboratories that require a ready-to-use, filter-sterilized formulation with common energy, buffering, and indicator components already included. The medium contains phenol red for visual monitoring of culture pH, 4.5 g/L D-glucose for high-glucose culture conditions, 4 mM L-glutamine as a nitrogen and energy-related nutrient, and 0.11 g/L sodium pyruvate as an additional carbon source.

The formulation is supplied without HEPES, making it suitable for incubator-based systems controlled by 5-10% CO2 at 37°C. Recommended use includes supplementation with 10% fetal bovine serum or another validated supplement system, depending on the cell type and internal protocol. Key release specifications include pH 7.0-7.4, osmolality 320-350 mOsmol/kg, endotoxin not more than 1 EU/mL, and sterility by filtration. The product is described as animal origin-free at the medium formulation level; serum or other supplements should be evaluated separately by the user.

• Routine culture of fibroblasts, smooth muscle cells, and other mammalian cell types that are compatible with high-glucose DMEM conditions.
• R&D studies requiring a phenol red-containing medium for rapid visual indication of pH shift during culture handling, incubation, and media exchange.
• Cell expansion workflows using 37°C incubation with 5-10% CO2, where the absence of HEPES is preferred to maintain standard bicarbonate and CO2-buffered culture conditions.
• Serum-supplemented protocols that specify 10% fetal bovine serum addition and require consistent medium background chemistry for lot-to-lot evaluation.
• Procurement programs consolidating classical liquid media, sera, and reagents with documented specifications and quotation support through request-quote workflows.

Store CCM-CM-0004 at 2-8°C and protect the bottle from light. Do not freeze unless the user has validated freezing and thawing for the specific application, because temperature cycling can affect medium appearance and performance. The stated shelf life is 12 months when stored unopened under recommended conditions. Shipments are handled on blue ice to maintain refrigerated transit conditions. Before use, inspect the liquid for clarity, color, leakage, or visible contamination, and warm only the required volume using a controlled laboratory procedure. Use aseptic technique after opening, and minimize repeated warming events or extended exposure to ambient light.

CellCultureMedia supplies CCM-CM-0004 for research, development, and bioprocess support environments that require traceable product data and procurement-ready documentation. Manufacturing and release testing follow ISO-aligned quality practices, with sterility, endotoxin, pH, osmolality, formulation, storage, and shelf-life information available for technical review. A Certificate of Analysis can be provided on request, and customers may contact our team for lot documentation, bulk supply planning, or specification confirmation through the quality resource page. Free worldwide shipping is available for qualifying orders, including 500 mL bottle quantities and larger purchasing schedules.