Waymouth MB 752/1 Medium with Phenol Red and L-Glutamine

CCM-CM-0037 is a ready-to-use liquid Waymouth MB 752/1 formulation supplied for laboratories that require a defined, reproducible medium for routine animal cell culture. The formulation includes phenol red for visual pH indication, 5.0 g/L D-glucose as a carbohydrate energy source, and 350 mg/L L-glutamine to support nitrogen metabolism in standard growth workflows. It is specified for use with L929 and comparable adherent cell systems, and source protocol data indicate operation with 10% FBS where the user’s method requires serum supplementation. The supplied medium itself is classified as animal-origin-free; added serum or other supplements should be assessed separately within the user’s quality system.

Key release specifications include sterile-filtered liquid format, pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin not more than 3 EU/mL, and a 12-month shelf life when stored as directed. These parameters make the product suitable for procurement teams standardizing classical media inputs across R&D, cell banking support, assay development, and scale-down process studies. For related formulations and comparable catalog items, see our classical media category.

• Routine expansion and maintenance of L929 cells and other compatible adherent animal cell lines under 37 °C, 5–10% CO2 culture conditions.
• Research workflows requiring a phenol-red-containing medium for rapid visual monitoring of culture pH shifts during incubation, feeding, or handling.
• Assay-ready culture systems where pH, osmolality, endotoxin, and sterile-filtration specifications must be documented for internal batch records.
• Small-scale bioprocess development, media comparison, and scale-down studies using a classical Waymouth MB 752/1 nutrient profile.
• Serum-supplemented workflows, including protocols that call for 10% FBS addition, with the final supplemented system qualified by the user.
• Academic, biotech, and biopharma research groups that need consistent 500 mL bottles with traceable procurement documentation.

Store unopened bottles at 2–8 °C protected from light. Do not freeze unless your internal validation supports freeze-thaw exposure, as temperature excursions can affect pH, solubility, and L-glutamine stability. Warm only the required volume to room temperature or incubation temperature before use, and handle aseptically in a certified biosafety cabinet to preserve sterility after opening. Shipping is performed on blue ice to maintain cold-chain conditions during transit; inspect the bottle on receipt for closure integrity, clarity, label match, and transport damage before releasing it for laboratory use.

CellCultureMedia supplies CCM-CM-0037 for research and bioprocessing customers that require specification-led ordering, documented QC, and responsive sourcing support. Manufacturing and release controls are ISO-aligned, with lot-level quality records available to support receiving inspection and internal qualification. A Certificate of Analysis can be requested through quality documentation support, including confirmation of sterility, endotoxin, pH, osmolality, shelf life, and applicable release criteria. For multi-bottle orders, standing supply, or project-based demand, submit a request for quote. Listed size is 500 mL, listed price is USD 145.83, and free worldwide shipping is available where import and lane conditions permit.