RPMI 1640 Powder Medium, 100 L

RPMI 1640 is a widely specified classical medium format for laboratory cell culture, assay development, and early process evaluation. CCM-CM-0040 is supplied as powder for customers who require 100 L preparation scale, reduced liquid freight volume, and control over final hydration, filtration, and supplementation procedures. The medium contains phenol red for visual pH indication and L-glutamine at 300 mg/L to support nitrogen metabolism in actively growing cultures. It excludes HEPES, making it suitable for laboratories that manage buffering primarily through bicarbonate and controlled CO2 incubation.

The formulation is classified as animal origin-free and is typically used with 10% FBS or an internally validated supplement system. Source operating conditions specify culture at 37 °C in 5–10% CO2. Key release specifications include endotoxin ≤1 EU/mL, osmolality 210–270 mOsmol/kg, and pH 6.0–6.6 for the powder-based product before customer-side final preparation. For related formats and comparable specifications, see our classical media category.

• Routine culture of RPMI-compatible suspension cell models used in immunology, hematology research, and cell biology workflows.
• PBMC handling, expansion studies, stimulation assays, and workflow development when the medium has been qualified by the user’s laboratory.
• Research-scale preparation of supplemented complete medium with 10% FBS or other validated supplement strategies.
• Method development where phenol red is useful for monitoring culture acidification during incubation.
• Procurement programs that require animal origin-free powdered medium, long shelf life, and consistent batch documentation.
• Laboratories seeking a HEPES-free RPMI 1640 base for CO2-controlled culture systems or custom buffer design.

Store the unopened powder at 2–8 °C protected from light. The stated shelf life is 48 months when stored under recommended conditions and handled according to good laboratory practice. Shipments are dispatched on blue ice where applicable to maintain temperature control during transit. After receipt, inspect packaging integrity, verify label details against the purchase order, and record lot information for traceability. During reconstitution, use validated water quality, mix until fully dissolved, adjust final pH and osmolality as required by your protocol, and perform sterile filtration before use unless your internal process specifies an alternative qualified preparation method.

CellCultureMedia supplies CCM-CM-0040 for research and bioprocess development buyers who need specification-driven ordering, lot traceability, and responsive documentation support. Manufacturing and release controls are ISO-aligned, with testing focused on identity, physical appearance, pH, osmolality, endotoxin, and lot consistency. A Certificate of Analysis is available on request, and additional documentation can be discussed through our quality documentation team. For standing orders, volume forecasts, or multi-site supply planning, submit a request through request a quote. Free worldwide shipping is available on eligible orders, and bulk pricing can be provided for 100 L powder formats and recurring procurement programs.