RPMI 1640 Medium with Phenol Red and L-Glutamine

CCM-CM-0012 is a sterile-filtered RPMI 1640 liquid medium for laboratories that require a defined, HEPES-free formulation with phenol red pH indicator and pre-added L-glutamine. The supplied medium is classified as animal-origin-free before user supplementation and is designed for routine cell culture in humidified incubators at 37 °C with 5–10% CO2. Phenol red supports visual monitoring of culture acidification, while 2.0 g/L D-glucose provides a standard carbon source for established RPMI 1640 workflows. The formulation is commonly paired with 10% fetal bovine serum, although final supplementation should be validated for each cell model and assay.

Key release specifications include pH 7.0–7.4, osmolality 260–305 mOsmol/kg, endotoxin ≤1 EU/mL, and sterility by filtration. This product is positioned in the classical media category for R&D, process development, and academic procurement teams that need consistent lot documentation and predictable ordering. The 500 mL bottle size supports small-batch experiments, method confirmation, and parallel culture evaluations without committing to larger-volume inventory.

• Maintenance and expansion of PBMC cultures and other suspension cell models that are routinely supported by RPMI 1640 chemistry.
• Culture of hematopoietic-derived research cell lines where a phenol-red-containing, L-glutamine-supplemented medium is specified by an internal protocol.
• Serum-containing assay development using user-added fetal bovine serum, growth supplements, or other qualified additives.
• CO2-buffered incubator workflows that do not require added HEPES and rely on bicarbonate buffering under controlled atmosphere conditions.
• Comparability studies, media screening, and procurement qualification for laboratories standardizing classical medium inputs across multiple teams.

Store CCM-CM-0012 at 2–8 °C and protect the bottle from light to preserve phenol red performance and overall formulation integrity. Do not freeze unless an internal stability protocol has been validated. The stated shelf life is 12 months when stored unopened under the recommended conditions. Handle using aseptic technique, inspect the bottle before use, and discard material that shows turbidity, leakage, visible particulates, or unexpected color shift. The product ships with blue ice to support refrigerated transit; upon receipt, transfer promptly to controlled cold storage and record the lot number for traceability.

CellCultureMedia supplies CCM-CM-0012 under ISO-aligned quality practices with batch-level release review for sterility, endotoxin, pH, osmolality, appearance, and labeling accuracy. A Certificate of Analysis can be provided on request, and procurement teams may contact us through request a quote for volume pricing, standing orders, or documentation needs. Quality expectations, shipping controls, and documentation support are summarized on our quality page. Free worldwide shipping is available for this SKU, helping international research organizations simplify landed-cost planning while maintaining cold-chain handling expectations.