RPMI 1640 Medium with HEPES and Pyruvate

This modified RPMI 1640 medium is a ready-to-use liquid formulation in the classical media range. It contains phenol red as a visual pH indicator, 4.5 g/L D-glucose, 300 mg/L L-glutamine, 0.11 g/L sodium pyruvate, equivalent to 1 mM, and 10 mM HEPES for additional buffering capacity. The formulation is specified as animal-origin-free before any user-added supplementation.

CCM-CM-0015 is intended for laboratories culturing suspension blood-derived models, PBMC preparations, and related research cell systems that commonly use RPMI 1640 chemistry. For complete culture preparation, the supplied specification indicates addition of 10% fetal bovine serum according to the user’s validated protocol. Release parameters include pH 7.0-7.4, osmolality 260-305 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter-sterilized liquid format.

• Routine expansion and maintenance of PBMC cultures in 37°C incubators with 5-10% CO2.
• Research culture of hematopoietic suspension cell models requiring RPMI 1640 nutrient balance and phenol red monitoring.
• Assay setup where HEPES buffering helps reduce short-term pH drift during handling outside the incubator.
• Process development, media comparison, and scale-up feasibility studies using a documented 500 mL liquid medium format.
• Workflows requiring high D-glucose, L-glutamine, and sodium pyruvate in a single preformulated base medium.

Store unopened bottles at 2-8°C protected from light. Do not freeze unless your internal validation has established acceptable performance after freezing. The stated shelf life is 12 months when stored under recommended conditions. Shipment is arranged on blue ice, and free worldwide shipping can be applied where route and order conditions allow. Bring the medium to working temperature using controlled laboratory procedures, mix gently, and use aseptic technique after opening. Supplement with serum or other additives only after confirming compatibility with the target cell system and protocol.

CellCultureMedia supplies CCM-CM-0015 under ISO-aligned manufacturing and release practices for research and bioprocessing customers. Lot-specific documentation can include sterility status, endotoxin, pH, osmolality, appearance, formulation identity, shelf life, and storage requirements. A Certificate of Analysis is available on request through the quality documentation team. For standing orders, multi-bottle requirements, or site-specific shipping documentation, contact us through request a quote so procurement and technical teams can confirm pricing, lead time, and delivery routing.