PK-15 Serum-Free ADCF Medium

This PK-15 serum-free ADCF medium is a ready-to-use liquid formulation for laboratories and process-development teams culturing PK-15 cells under defined, animal-origin-free conditions. The medium is supplied with phenol red for visual pH monitoring, D-glucose as the carbohydrate source, 4 mM L-glutamine, and 2 mM L-alanyl-L-glutamine to support glutamine availability during routine culture operations. It is intended for incubator conditions of 37 °C and 5–10% CO2.

Key release specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin ≤3 EU/mL, and sterile-filtered liquid presentation. The serum-free classification reduces variability associated with serum supplementation and supports more consistent upstream workflow design. The animal-origin-free profile is suitable for organizations that require documented material controls for research, scale-up, and biopharmaceutical process development. Each order is identified as CCM-BP-0001 for procurement traceability.

• Maintenance and expansion of PK-15 cells in serum-free culture systems.
• Feasibility studies comparing animal-origin-free media against serum-containing legacy processes.
• Upstream process development where defined composition, osmolality control, and low endotoxin specifications are required.
• Bioreactor, spinner, shake-flask, and static-culture screening after internal adaptation and performance qualification.
• Research workflows supporting vaccine-process development and other biopharmaceutical production platforms, without reliance on serum supplementation.
• Procurement programs standardizing 500 mL liquid media formats for multi-site R&D teams.

Store unopened bottles at 2–8 °C and protect from light. The stated shelf life is 12 months when stored as recommended and handled within the assigned expiry dating. This product ships on blue ice to help maintain cold-chain conditions during transit; inspect packaging on arrival and transfer the bottle promptly to refrigerated storage. Use aseptic technique after opening, minimize repeated warming, and equilibrate only the required volume before use. Do not use if the container is compromised, the liquid shows unexpected turbidity, or internal acceptance criteria are not met.

CellCultureMedia supplies this medium for research and bioprocessing use with ISO-aligned manufacturing controls, sterile filtration, and specification-based lot release. Lot-specific documentation can be requested through the quality documentation pathway, including Certificate of Analysis availability for pH, osmolality, sterility, endotoxin, and other applicable release tests. For standing orders, multi-lot planning, or site-specific documentation requirements, procurement teams can request a quote. Free worldwide shipping is available, and shipping conditions are selected to match the refrigerated storage requirement.