MDCK Suspension Serum-Free Medium

CCM-BP-0004 is a ready-to-use liquid medium designed for serum-free MDCK suspension culture where defined composition, lot consistency, and simplified downstream processing are procurement priorities. The product is animal-origin-free and sterile-filtered, supporting workflows that require reduced adventitious-material risk and clear documentation. It is supplied in a 500 mL format at $319.44 per bottle and fits R&D, seed-train, scale-down, and pilot bioprocess evaluation programs.

The medium contains phenol red for visual monitoring of pH drift, D-glucose as a primary carbon source, 4 mM L-glutamine, and 2 mM L-alanyl-L-glutamine for additional glutamine stability in culture. Release specifications include pH 7.0–7.4 and osmolality of 270–340 mOsmol/kg, supporting typical mammalian cell culture operating ranges. Endotoxin is specified at ≤3 EU/mL, and the product is sterile-filtered before release. For related defined liquid media, see our bioprocess media category.

• Suspension culture of MDCK cells at 37 °C in incubators or controlled bioreactor systems operated with 5–10% CO2.
• Serum-free process development where reduction of serum-associated variability is required for media screening, scale-down modeling, and seed expansion.
• Animal-origin-free biopharmaceutical manufacturing support workflows, including upstream process characterization and viral antigen production research.
• Comparability studies in which pH, osmolality, endotoxin, sterility, and defined additive profiles must be documented for batch records.
• Procurement programs that need a 500 mL pack size for qualification runs before requesting larger recurring supply. For volume requirements, submit details through request a quote.

Store unopened bottles at 2–8 °C and protect from light to preserve phenol red performance and formulation integrity. Do not freeze unless your internal validation program has qualified freeze-thaw impact for this formulation. Use aseptic technique when opening and dispensing, and minimize repeated warming cycles by aliquoting when appropriate. The stated shelf life is 12 months when stored under recommended conditions. Before use, equilibrate only the required volume to culture temperature and confirm that the medium remains clear and within expected color range.

CellCultureMedia supplies CCM-BP-0004 for research, development, and bioprocessing environments that require specification-driven purchasing. Manufacturing and release controls are ISO-aligned, with sterility, pH, osmolality, endotoxin, shelf-life, storage, and formulation attributes available for procurement review. A Certificate of Analysis can be requested for the supplied lot, and additional documentation questions can be directed through our quality information channel. Orders include free worldwide shipping where import regulations and temperature-control logistics permit; regional lead times are confirmed at quotation or order acknowledgment.