MEM Powder with Phenol Red and Earle’s Salts

CCM-CM-0041 is an animal origin-free MEM powder for users who need to prepare consistent volumes of classical medium in-house. The formulation includes phenol red as a pH indicator, 1.0 g/L D-glucose as the carbohydrate source, 2 mM L-glutamine, MEM non-essential amino acids, and Earle’s balanced salts. This composition supports common mammalian cell culture workflows using a 10% FBS supplementation strategy, with incubation typically performed at 37°C in 5–10% CO2 depending on the cell type and laboratory protocol.

The product is specified as powder with pH 4.5–5.5 before final use adjustment and osmolality of 230–275 mOsmol/kg after preparation according to validated procedures. Endotoxin is specified at ≤1 EU/mL, and the listed shelf life is 48 months when stored as directed. For related formulations and comparison options, review our classical media portfolio or contact CellCultureMedia with your media panel, volume plan, and documentation requirements.

• Routine culture of fibroblasts and similar adherent mammalian cell types when supplemented according to the user’s validated serum and process conditions.
• Culture of primary rat astrocytes and other primary research cell preparations that use MEM-based classical medium designs.
• Basic research workflows requiring phenol red for visual pH monitoring during culture setup, handling, and incubation.
• Laboratory-scale and pilot-scale medium preparation where a powder format reduces storage burden and supports batch preparation of up to 100 L.
• Comparability studies, formulation screening, and process development work where MEM with Earle’s salts, L-glutamine, and non-essential amino acids is the required baseline composition.
• Procurement programs that need an animal origin-free classification, defined endotoxin limit, osmolality range, shelf-life statement, and lot documentation for internal release review.

Store the powder at 2–8°C protected from light, and keep the container closed until preparation to limit moisture uptake and light exposure. The material ships on blue ice to support temperature-controlled transit. Reconstitute only with suitable cell culture-grade water and perform final preparation, filtration, supplementation, and pH confirmation under your site’s approved procedure. Because this formulation contains L-glutamine and phenol red, avoid unnecessary warming, repeated exposure to room light, or extended holding of prepared liquid medium outside validated conditions. Assign prepared-medium hold times, sterility checks, and release criteria according to your internal quality system.

CellCultureMedia supplies CCM-CM-0041 for scientific and basic research use, with specification-driven documentation to support purchasing, receiving inspection, and laboratory release. Manufacturing and quality controls are ISO-aligned, and lot-specific Certificate of Analysis documentation is available on request through our quality team. Typical documentation may include product identity, appearance, pH, osmolality, endotoxin limit, storage, shelf life, and applicable release data. For volume planning, standing orders, or multi-site supply coordination, submit a request through request a quote. The 100 L size is listed at $1,045.83, and free worldwide shipping is available where service and temperature-control lanes support the shipment.