MEM Alpha Medium, No Nucleosides or Phenol Red, with L-Glutamine

MEM Alpha Medium without nucleosides or phenol red is a classical cell culture medium for applications that require tight control over nucleoside availability and reduced interference from pH-indicator dye. This animal origin-free liquid formulation contains 1.0 g/L D-glucose and 2 mM L-glutamine, while excluding phenol red, nucleosides, and deoxynucleosides. It is supplied sterile-filtered in a 500 mL bottle under SKU CCM-CM-0027 for research and bioprocessing use.

The medium is specified at pH 7.0-7.4 and osmolality 280-330 mOsmol/kg, with endotoxin controlled to ≤1 EU/mL. It is commonly used as a base formulation for keratinocytes, human melanoma research cell lines, and other adherent or suspension models where protocol-defined supplementation is required. Many workflows use it with 10% FBS or other laboratory-selected supplements, depending on the cell type and experimental design. See related classical media options for matched formulations and alternative indicator or nucleoside profiles.

• Culture of keratinocyte models and other epithelial research cells requiring MEM Alpha nutritional support.
• Expansion and maintenance of human melanoma research cell lines in protocols that specify medium without added nucleosides.
• Assay systems where phenol red is omitted to reduce background in optical, fluorescence, absorbance, or hormone-sensitive readouts.
• Controlled supplementation studies using user-defined serum, growth factor, lipid, or small-molecule additions.
• Comparative media screening where glucose, L-glutamine, pH, osmolality, and endotoxin specifications must be consistent across lots.
• Academic, biotech, and biopharma R&D programs that require a documented 500 mL liquid medium with animal origin-free classification.

Store CCM-CM-0027 at 2-8 °C and protect the bottle from light throughout storage and routine laboratory use. Do not freeze unless the receiving laboratory has validated freeze-thaw compatibility for its own workflow. The stated shelf life is 12 months when the product remains unopened and stored under recommended conditions. Shipments are dispatched on blue ice to support temperature-controlled transit; inspect bottles on receipt, record condition and lot information, and equilibrate only the required working volume to room temperature before use. Apply aseptic technique after opening and avoid repeated warming cycles that can reduce medium performance.

CellCultureMedia supplies this medium through ISO-aligned manufacturing and release processes with specification checks for sterility, pH, osmolality, endotoxin, appearance, and formulation identity. A Certificate of Analysis can be requested for lot-specific procurement records, incoming QC, and audit files through our quality documentation channel. The product is intended for scientific and basic research applications only. For standing orders, multi-bottle purchases, or institution-level supply agreements, request pricing and availability through the quote request page. Free worldwide shipping is available, with blue-ice packing used for this temperature-sensitive liquid medium.