MEM Alpha Medium with Phenol Red, Glutamine and Nucleosides

CCM-CM-0028 is an MEM Alpha liquid medium configured for routine expansion and maintenance of mammalian cells in carbon dioxide incubators. The medium contains phenol red for visual pH monitoring, 2 mM L-glutamine as a nitrogen and energy source, 1.0 g/L D-glucose, nucleosides, and deoxynucleosides. These components support nucleotide metabolism and general growth requirements in serum-supplemented culture systems. It is supplied as a ready-to-use liquid base medium and should be completed with 10% fetal bovine serum or an internally qualified supplement strategy before use.

The specified operating profile is 37 °C with 5-10% CO2, with a finished medium pH of 7.0-7.4 and osmolality of 280-330 mOsmol/kg. The product is filter sterilized, specified with endotoxin at ≤1 EU/mL, and classified as animal-origin-free before serum supplementation. It is suitable for procurement programs standardizing classical media inputs across research, screening, and early process development workflows. For related formulations, see the classical media category.

• Serum-supplemented culture of keratinocytes and human melanocytic research models where MEM Alpha chemistry has been internally qualified.
• Routine mammalian cell maintenance, passaging, and expansion in academic, biotech, and biopharma R&D laboratories.
• Comparability studies where phenol red, L-glutamine, nucleosides, and deoxynucleosides are required in the base formulation.
• Cell culture method development under 37 °C and 5-10% CO2 conditions with controlled pH and osmolality specifications.
• Inventory consolidation for laboratories requiring a 500 mL, sterile, liquid classical medium with documented endotoxin and shelf-life attributes.

Store unopened bottles at 2-8 °C protected from light. Do not freeze, and minimize warming cycles to preserve component integrity, especially L-glutamine and phenol red performance. The stated shelf life is 12 months when stored under recommended conditions. Shipments are packed on blue ice to maintain refrigerated transit conditions. Before use, inspect the bottle for clarity, closure integrity, and absence of visible contamination. Warm only the volume needed for the current operation, mix gently, and use aseptic technique when adding serum or other qualified supplements.

Each lot is manufactured under ISO-aligned quality practices with sterile filtration and release testing appropriate for research and bioprocessing supply. Key specifications include pH 7.0-7.4, osmolality 280-330 mOsmol/kg, endotoxin ≤1 EU/mL, 12-month shelf life, and refrigerated light-protected storage. A Certificate of Analysis can be provided on request, and lot traceability is supported for purchasing teams managing approved supplier files. Review CellCultureMedia quality expectations at quality documentation or submit volume requirements through request a quote. Free worldwide shipping is available where import and lane conditions allow refrigerated delivery.