M199 Powder with Phenol Red and L-Glutamine

CCM-CM-0042 is M199 in powder form with phenol red, Earle's balanced salts, and L-glutamine included. The formulation excludes sodium bicarbonate and HEPES, making it suitable for laboratories that prefer to set bicarbonate concentration, final pH, filtration workflow, and gas-phase equilibration according to their own SOPs. The supplied pack size is configured for 100 L of finished medium when reconstituted according to validated internal procedures.

This medium is intended for scientific and basic research cell culture workflows, including chicken embryo fibroblast culture and other compatible animal cell systems that require M199 chemistry. Source specifications indicate use with 10% FBS and incubation at 37 °C under 5–10% CO2 after preparation. Key release parameters include pH 4.3–4.9 for the supplied powder specification, osmolality target of 240–300 mOsmol/kg after preparation, endotoxin not more than 1 EU/mL, and a 48-month shelf life when stored as directed. For adjacent formulations, see the classical media category.

• Preparation of M199-based complete medium for chicken embryo fibroblasts and related adherent animal cell culture models.
• Research and basic science workflows requiring phenol red as a visual pH indicator during routine culture monitoring.
• CO2-incubator processes where sodium bicarbonate is added separately to align the final buffer system with local culture conditions.
• Formulation development studies comparing bicarbonate concentration, serum level, and process pH under controlled 37 °C, 5–10% CO2 conditions.
• Procurement-standardized stocking for laboratories that need a dry medium format with long shelf life and reduced liquid-media storage volume.
• Scale-aware preparation of up to 100 L per unit for R&D groups, pilot-scale cell culture rooms, and academic core facilities.

Store the unopened powder at 2–8 °C protected from light. The stated shelf life is 48 months when storage conditions are maintained and the container remains sealed. Shipments are arranged on blue ice where required to maintain controlled transit conditions. During preparation, use qualified water and validated mixing procedures, then add sodium bicarbonate, serum, and other supplements as required by the cell system. Because this product contains L-glutamine, minimize unnecessary heat exposure and prolonged hold times after reconstitution. Final pH, osmolality, and sterility should be verified according to the user's internal release criteria before introducing the medium to cell cultures.

CellCultureMedia supplies CCM-CM-0042 for research and bioprocess development use with procurement documentation suitable for institutional purchasing. Manufacturing and lot release are managed under ISO-aligned quality practices, with specifications covering powder form, endotoxin limit, osmolality target, pH range, storage condition, and shelf-life assignment. A Certificate of Analysis is available on request, and additional documentation can be routed through quality support for supplier qualification files. The listed 100 L unit is priced at $1,254.17, with free worldwide shipping available for eligible orders. For multi-unit demand, standing purchase planning, or matched-lot requirements, submit a request through the CellCultureMedia quotation workflow.