M16 Embryo Culture Medium

M16 Embryo Culture Medium is part of the CellCultureMedia classical media portfolio for laboratories that require a defined, ready-to-use liquid medium for embryo culture studies, gamete handling protocols, and related mammalian developmental biology research. The product is classified as animal-origin and is supplied in a 50 mL bottle for low-volume, high-control workflows where lot traceability, defined specifications, and consistent replenishment matter to procurement and laboratory teams.

The chemistry is built around a bicarbonate-buffered system, making the medium appropriate for use in a controlled CO2 incubator environment. Sodium lactate is included as a carbon and energy source, sodium bicarbonate provides buffering capacity, and phenol red supports visual pH monitoring during routine manipulation. The medium is manufactured without HEPES and without penicillin-streptomycin, allowing scientists to manage buffering strategy and antimicrobial supplementation separately when their validated protocol requires it. Release specifications include pH 7.0-7.6, osmolality 280-304 mOsmol/kg, endotoxin ≤10 EU/mL, and a stated shelf life of 12 months when stored as directed.

• Research culture of mammalian embryos in bicarbonate-buffered incubator conditions.
• Mouse embryo handling and short-term culture workflows in academic and biopharma R&D laboratories.
• Protocol-controlled studies requiring sodium lactate, sodium bicarbonate, and phenol red in a classical medium format.
• Workflows where HEPES-free medium is preferred to keep buffering dependent on CO2 and bicarbonate equilibrium.
• Antibiotic-free culture systems where penicillin-streptomycin is excluded to support investigator-defined experimental design.
• Lot-controlled purchasing programs for laboratories standardizing embryo culture reagents across multiple users or project teams.

Store M16 Embryo Culture Medium at 2-8 °C protected from light. Do not expose the bottle to repeated warming cycles, prolonged room-temperature holding, or direct illumination. The product ships with blue ice to support refrigerated transit conditions; upon receipt, inspect the bottle, label, and package integrity before placing the medium into controlled cold storage. For best practice, equilibrate only the working volume required by the protocol, use aseptic technique, and avoid returning warmed or opened aliquots to the original container. The declared shelf life is 12 months under recommended storage conditions.

CellCultureMedia supplies CCM-CM-0044 for research and bioprocess development procurement channels that require clear specifications, stable documentation, and responsive quotation support. Manufacturing and release workflows are ISO-aligned, and lot-specific documentation can be requested through the quality documentation team, including a Certificate of Analysis when available for the shipped lot. The listed 50 mL pack is priced at USD 194.44, with free worldwide shipping available on eligible orders. For standing orders, multi-lot planning, or institutional purchasing, submit requirements through request a quote so our team can confirm availability, lead time, and volume pricing.