Low-Glucose DMEM with Phenol Red, Glutamine and Pyruvate

CCM-CM-0011 is a low-glucose Dulbecco's Modified Eagle Medium for cell culture workflows requiring a defined classical medium with phenol red as a visual pH indicator. The formulation includes 1.0 g/L D-glucose, 4 mM L-glutamine, and 0.11 g/L sodium pyruvate, and it is manufactured without HEPES. The reduced glucose level is commonly selected for cell types and experimental designs where high-glucose conditions are not desired. Sodium pyruvate provides an additional carbon and energy substrate, while L-glutamine supports nitrogen metabolism in standard mammalian culture systems.

This liquid medium is intended for scientific and basic research use in laboratories, core facilities, and process-development groups. Typical use requires supplementation with 10% fetal bovine serum or an equivalent validated supplement according to the user's cell line protocol. Key release specifications include pH 7.0–7.4, osmolality 310–340 mOsmol/kg, endotoxin ≤1 EU/mL, and filtration-based sterility processing. Procurement teams can use these values for incoming material checks, lot comparison, and documentation alignment across multi-site cell culture operations.

• Routine culture of fibroblasts, smooth muscle cells, and other adherent mammalian cells compatible with low-glucose DMEM chemistry.
• Research-scale expansion, maintenance, and assay preparation where phenol red is useful for visual monitoring of medium color shift during incubation.
• CO2 incubator culture at 37 °C with 5–10% CO2, using validated serum supplementation, commonly 10% fetal bovine serum.
• Comparative formulation studies where a HEPES-free medium is required to control buffering variables under bicarbonate and CO2 conditions.
• Academic, biotechnology, and biopharma R&D workflows requiring a ready-to-use, filter-sterilized classical medium with documented pH, osmolality, and endotoxin limits.

Store CCM-CM-0011 at 2–8 °C protected from light. The product is shipped on blue ice to help maintain refrigerated conditions during transit, with free worldwide shipping available for eligible orders. Do not freeze unless the receiving laboratory has validated freeze-thaw handling for this specific formulation. Use aseptic technique after opening, minimize repeated warming cycles, and record bottle opening dates in accordance with internal quality procedures. The stated shelf life is 12 months when unopened and stored under the recommended conditions. Because the medium contains L-glutamine and phenol red, prolonged exposure to light, elevated temperature, or non-sterile handling may affect performance characteristics.

CellCultureMedia supplies CCM-CM-0011 for research and bioprocessing environments that require traceable, specification-driven cell culture materials. Manufacturing and release practices are ISO-aligned, and lot documentation can be provided to support receiving inspection, audit files, and change-control review. A Certificate of Analysis is available on request and may include release data such as appearance, pH, osmolality, sterility status, endotoxin level, and shelf-life information. For procurement planning, request a quotation through request quote with expected volume, delivery location, and recurring demand. Additional information about documentation practices is available on the quality page.