High Glucose DMEM with Phenol Red, Glutamine and Pyruvate

CCM-CM-0005 is a high glucose Dulbecco’s Modified Eagle Medium configuration for research and bioprocess development laboratories that need a defined, procurement-ready liquid medium. The formulation includes phenol red for visual pH indication, 4.5 g/L D-glucose as the carbohydrate source, L-glutamine as a nitrogen and energy-related nutrient, and sodium pyruvate as an additional metabolic substrate. HEPES is not included, so the medium is designed for incubation in a controlled CO2 environment using the bicarbonate buffer system.

The product is supplied as a 500 mL sterile-filtered liquid with a specified pH range of 7.0–7.4 and osmolality of 260–320 mOsmol/kg. Endotoxin is specified at ≤1 EU/mL, supporting use in controlled cell culture studies where lot documentation and repeatable input materials are important. The medium is classified as animal-origin-free in its supplied form; users may add 10% FBS or an alternative supplement if required by the cell model. For related formulations and alternative glucose or additive profiles, see our classical media category.

• Routine culture of fibroblasts, smooth muscle cells, and other adherent mammalian cell types that are compatible with high glucose DMEM chemistry.
• Research-scale expansion, maintenance, and experimental setup in incubators operated at 37 °C with 5–10% CO2.
• Serum-supplemented workflows using a typical 10% FBS level, as well as internally qualified supplement systems selected by the laboratory.
• Comparative media screening where phenol red, L-glutamine, sodium pyruvate, and no HEPES are required as fixed formulation parameters.
• Basic research, assay preparation, and upstream process development studies requiring a documented liquid medium with defined pH, osmolality, sterility, shelf-life, and endotoxin specifications.

Store CCM-CM-0005 at 2–8 °C protected from light. Do not use the product beyond its assigned 12-month shelf life, and minimize repeated warming cycles to maintain formulation consistency. The medium is shipped on blue ice and should be inspected on receipt for container integrity, labeling, and acceptable temperature condition. Equilibrate only the required volume to working temperature before use, and follow aseptic technique when opening, aliquoting, or supplementing the bottle. Because the formulation does not contain HEPES, maintain appropriate CO2 control during incubation to keep pH within the intended operating range.

CellCultureMedia supplies CCM-CM-0005 for B2B research, academic, and bioprocessing procurement programs requiring traceable media inputs. Manufacturing and release controls are ISO-aligned, and lot-specific quality documentation can be provided on request, including Certificate of Analysis details for sterility, endotoxin, pH, osmolality, shelf life, and product identity. For documentation expectations, visit our quality information page. The listed 500 mL price is USD 145.83, with free worldwide shipping available for eligible orders. For standing orders, multi-bottle requirements, or institutional purchasing, submit details through request a quote for lead-time and volume pricing review.