High-Glucose DMEM with Phenol Red, Glutamine and HEPES

High-glucose DMEM is a widely used nutrient medium for adherent and suspension-adapted mammalian cell culture systems. This formulation contains phenol red for visual pH monitoring, 4.5 g/L D-glucose for high-energy culture conditions, 4 mM L-glutamine as a nitrogen and energy source, and HEPES for supplemental buffering. Sodium pyruvate is intentionally excluded for users who prefer to control pyruvate addition separately or compare pyruvate-free culture conditions. For related formulations, see our classical media portfolio.

CCM-CM-0007 is supplied as a liquid, animal-origin-free medium before user supplementation. Typical use includes supplementation with 10% FBS when required by the cell model or laboratory protocol. Key release specifications include pH 7.0–7.4, osmolality 295–340 mOsmol/kg, endotoxin ≤1 EU/mL, and sterile-filtered processing. The recommended culture environment is 37 °C with 5–10% CO2, depending on vessel format, incubator settings, and supplementation strategy. The 500 mL bottle size supports routine R&D use, media screening, and controlled small-batch production planning.

• Routine culture of fibroblasts, smooth muscle cells, and other mammalian cell types commonly maintained in high-glucose DMEM-based systems.
• Research workflows requiring phenol red for rapid visual assessment of medium pH changes during incubation, feeding, and passaging.
• Protocols where L-glutamine is included in the base formulation and sodium pyruvate is omitted for controlled experimental design.
• CO2 incubator culture at 37 °C with 5–10% CO2, including flask, plate, roller bottle, and selected closed-vessel formats.
• Serum-supplemented culture using 10% FBS where required by the cell line, with user-defined additions such as antibiotics, growth supplements, or attachment factors.
• Media comparison studies, process development screening, and academic or industrial cell biology projects where pH, osmolality, sterility, and endotoxin specifications must be documented.

Store CCM-CM-0007 at 2–8 °C protected from light. Do not freeze, and minimize repeated warming cycles to help maintain formulation consistency. The product is shipped on blue ice and should be transferred to refrigerated storage promptly upon receipt. Before use, inspect the bottle for seal integrity, clarity, and absence of particulates; warm only the required volume using controlled laboratory practice. The stated shelf life is 12 months when unopened and stored under recommended conditions. After opening, handle aseptically in a suitable biosafety cabinet or controlled clean area and follow internal procedures for in-use dating, contamination monitoring, and disposal.

CellCultureMedia supplies CCM-CM-0007 for organizations that need consistent procurement, documented specifications, and responsive quotation support. Manufacturing and release controls are ISO-aligned, with sterile filtration, endotoxin testing, pH verification, and osmolality testing included in the quality framework. A lot-specific Certificate of Analysis is available on request through our quality documentation team. For multi-bottle orders, recurring demand, or site-standardization projects, request pricing through our quotation portal. Free worldwide shipping is available, and bulk pricing can be reviewed for consolidated purchasing programs.