High-Glucose DMEM with L-Glutamine and HEPES

CCM-CM-0008 is a high-glucose Dulbecco’s Modified Eagle Medium supplied in ready-to-use liquid form for users who require a phenol red-free and sodium pyruvate-free formulation. The formulation includes 4.5 g/L D-glucose for high-energy culture conditions, 4 mM L-glutamine as a nitrogen and carbon source, and HEPES for enhanced buffering during routine handling outside the incubator. It is intended for scientific and basic research applications, including mammalian cell expansion, assay preparation, and process-development studies.

This medium is part of the CellCultureMedia classical media portfolio and is suitable for laboratories standardizing DMEM-based workflows across R&D, academic, and biopharma environments. The unsupplemented medium is animal-origin-free; typical use requires addition of 10% fetal bovine serum or an internally qualified alternative supplement. Source specifications include sterile filtration, pH 7.0–7.4, osmolality 295–340 mOsmol/kg, endotoxin ≤1 EU/mL, and a 12-month shelf life when stored correctly. Because the formula excludes phenol red, it is appropriate where visible pH indicator dye may interfere with absorbance, fluorescence, imaging, or color-sensitive readouts. Because it excludes sodium pyruvate, users can control pyruvate concentration independently when comparing metabolic conditions or optimizing proprietary culture protocols.

• Routine culture of fibroblasts, smooth muscle cells, and other validated mammalian cell types maintained in high-glucose DMEM conditions.
• Serum-supplemented culture systems requiring user addition of 10% fetal bovine serum or a qualified supplement according to the laboratory’s protocol.
• Assay workflows that benefit from a phenol red-free background, including plate-based optical measurement, microscopy, reporter analysis, and cell-density monitoring.
• Research studies where sodium pyruvate must be omitted from the base medium so that carbon-source variables can be evaluated separately.
• 37 °C incubator operation with 5–10% CO2, consistent with the stated culture environment for this formulation.
• Process development, media comparison, incoming-material qualification, and scale-up feasibility work where defined pH, osmolality, endotoxin, sterility, and lot documentation are procurement requirements.

Store CCM-CM-0008 at 2–8 °C protected from light. Do not freeze unless your internal validation permits freeze-thaw exposure, as freezing can affect salts, buffering performance, and soluble components in complete media workflows. The product ships on blue ice and should be transferred to refrigerated storage upon receipt. Use aseptic technique when opening the bottle, minimize repeated warming, and close the container promptly after dispensing. The specified shelf life is 12 months under recommended storage conditions; always follow the lot-specific expiry date on the label and record opening dates for controlled inventory management.

CellCultureMedia supplies CCM-CM-0008 for research and bioprocessing use with procurement-focused specifications suitable for vendor qualification and internal material review. Manufacturing and release controls are aligned with ISO-style quality management expectations for cell culture reagents, including filtration-based sterility processing and lot traceability. A Certificate of Analysis is available on request and may include lot-specific release data such as pH, osmolality, endotoxin, appearance, and sterility status. Buyers can review documentation practices through our quality information page or request a quote for bulk quantities, scheduled deliveries, and multi-site purchasing programs. Free worldwide shipping is available for this 500 mL format, with cold-chain packing selected for temperature-sensitive transit.